[1]杨庆胜,李彩荣,赵雪晖.HPLC-ELSD测定补益蒺藜片中黄芪甲苷的含量[J].甘肃中医药大学学报,2005,(01):42-44.
 Yang Qingsheng,Li Cairong,Zhao Xuehui.Using HPLC-ELSD to determine the sarcosine of astragalus root content of invigorated tribulus fruit[J].Journal of Gansu University of Chinese Medicine,2005,(01):42-44.
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HPLC-ELSD测定补益蒺藜片中黄芪甲苷的含量()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
期数:
2005年01期
页码:
42-44
栏目:
中药研究
出版日期:
1900-01-01

文章信息/Info

Title:
Using HPLC-ELSD to determine the sarcosine of astragalus root content of invigorated tribulus fruit
作者:
杨庆胜 李彩荣 赵雪晖
河南仲景保健药业有限公司, 河南 郑州 450001
Author(s):
Yang Qingsheng Li Cairong Zhao Xuehui
Zhongjing Health Protective Medical Limited Company in He’nan, 450001, Zhengzhou, China
关键词:
补益蒺藜片黄芪甲苷高效液相-蒸发光散射检测法含量测定
Keywords:
invigorated tribulus fruitastrag alinHPLC-ELSDdetermining content
分类号:
R927.2
摘要:
目的:建立补益蒺藜片中黄芪甲苷的含量测定方法。方法:采用高效液相-蒸发光散射检测法(HPLC-ELSD),对补益蒺藜片中黄芪甲苷进行含量测定。色谱柱为ODS-C18色谱柱(250 mm×4.6 mm,20μm);流动相:乙腈-水(1:2);进样量10μL;室温;流速1.0 mL/min;ELSD参数:漂移管温度100℃,气体流速2.74 mL/min。结果:黄芪甲苷对照品在2.024~10.120μg范围内,进样量与峰面积间呈良好的线性关系,r=0.9996,平均回收率为99.26%。结论:本方法操作简便,结果准确,重现性好,可用于控制该制剂的质量。
Abstract:
Objective: To establish determining method of the astragalin content in invigorated tribulus fruit. Methods: Adopting HPLC-ELSD, the astragalin content of invigorated tribulus fruit was determined. ODS-C18 chromatog raphic column (250 mm ×4.6 mm, 20 μm) was chosen, the mobile phase was acetonitrile: heavy distilled water (1:2), the sample input was 10 μL, the column was at a room temperature, the flow rate was 1.0 mL per minute, the temperature of drift duct was 100 centig rade degree, the flow rate of air was 2.74 mL per minute. Results: The control content of astragalin was within the range of 2.024 μg and 10.12 μg, and there was the fine linear relation ship between the sample input and the peak area, the average recovery was 99.26 percent. Conclusion: This operational method is simple, accurate and has good reappearance, and can be used to control the quality of this pharmaceutics.

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备注/Memo

备注/Memo:
收稿日期:2004-08-03
作者简介:杨庆胜(1969-),男,主管中药师,学士学位,主要从事中药的成分分析与新产品开发。
更新日期/Last Update: 1900-01-01