[1]杨树声,宋平顺,赵建邦,等.HPLC法同时测定丹参类药材中水溶性活性成分的含量[J].甘肃中医药大学学报,2008,(06):42-45.
 Yang Shusheng,Song Pingshun,Zhao Jianbang,et al.Simultaneous determination of water-soluble active components in Salvia miltiorrhiza Bge. and Salvia przewalskii Maxim. by HPLC[J].Journal of Gansu University of Chinese Medicine,2008,(06):42-45.
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HPLC法同时测定丹参类药材中水溶性活性成分的含量()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
期数:
2008年06期
页码:
42-45
栏目:
技术与方法
出版日期:
1900-01-01

文章信息/Info

Title:
Simultaneous determination of water-soluble active components in Salvia miltiorrhiza Bge. and Salvia przewalskii Maxim. by HPLC
作者:
杨树声1 宋平顺2 赵建邦2 丁永辉3
1. 定西市人民医院, 甘肃 定西 743000;
2. 甘肃省药品检验所甘肃省中药分析测试中心, 甘肃 兰州 730000;
3. 甘肃省食品药品监督管理局, 甘肃 兰州 730000
Author(s):
Yang Shusheng1 Song Pingshun2 Zhao Jianbang2 Ding Yonghui3
1. City of Dingxi People’s Hospital, Dingxi 743000, China;
2. Gansu Institute for Drug Control, Lanzhou 730000, China;
3. Gansu Food and Drug Administration, Lanzhou 730002, China
关键词:
高效液相色谱法丹参甘肃丹参水溶性成分丹参素钠原儿茶醛丹酚酸B栽培野生
Keywords:
HPLCSalvia miltiorrhiza BgeSalvia przewalskii Maxim.water-soluble componentssodium danshensuprotocatechuialdehydesalvianolic acid Bcultivatedwild
分类号:
R284.1
摘要:
目的 建立HPLC法同时测定丹参和甘肃丹参中丹参素钠、原儿茶醛和丹酚酸B含量的方法,通过3种水溶性活性成分测定,评价野生和甘肃栽培丹参、野生和栽培甘肃丹参的质量。方法 色谱柱为C18色谱柱(4.6 mm×250 mm,5 μm);流动相为甲醇-冰醋酸-水(20:80:1);流速1.0 mL/min。二元梯度洗脱。结果 丹参素钠、原儿茶醛、丹酚酸B分别在58~1455 ng、15~162 ng、825~8250 ng范围内线性关系良好,回收率分别为102.20%、101.53%和103.03%。结论 方法简便、准确、分离效果好,可作为丹参水溶性活性3种成分的同时定量测定方法。
Abstract:
Objective To develop an HPLC method on the simultaneous determination of sodium danshensu, protocatechuialdehyde, salvianolic acid B in Salvia miltiorrhiza Bge. and Salvia przewalskii Maxim. Evaluated the quality of wild and cultivated Salvia miltiorrhiza Bge. and Salvia przewalskii Maxim. by determining three kinds of water-soluble active components in them.Methods Use the method dual gradient elution. The analysis was carried on a chromatographic C18(4.6 mm×250 mm, 5 μm) with a mobile phase of methanol-glacial acetic acid-water(20:80:1) at the flow rate of 1.0 mL/min.Results The linear correlation of sodium danshensu, protocatechuialdehyde, salvianolic acid B were well in 58~1455 ng,15~162 ng and 825~8250 ng respectively. The average recoveries were 102.20%,101.53% and 103.03% respectively.Conclusion This method is convenient, accurate and rapid. It can be used to determine the three water-soluble active components in Salvia miltiorrhiza Bge. and Salvia przewalskii Maxim. at the same time.

参考文献/References:

[1] 国家药典委员会.中国药典(2005年版一部)[M].北京:化学工业出版社,2005:58.
[2] 赵建邦.甘肃丹参的研究与应用评价[J].中药材,2003,26(7):581.
[3] 王宇红,张水寒,杨永华,等.丹参超微片水溶性有效部位的HPLC指纹图谱研究[J].中成药,2007,29(4):480.

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备注/Memo

备注/Memo:
收稿日期:2008-06-12;改回日期:2008-09-21。
作者简介:杨树声(1964-),男,副主任药师,理学学士,主要从事中药材的检验与鉴定工作。
更新日期/Last Update: 1900-01-01