[1]刘雄,吴蓉,余晓晖,等.HPLC法测定不同产地肉苁蓉中松果菊苷的含量[J].甘肃中医药大学学报,2009,(05):42-44.
 Liu Xiong,Wu Rong,Yu Xiaohui,et al.Determination of echinacoside in Herba Cistanchis from different regions by HPLC[J].Journal of Gansu University of Chinese Medicine,2009,(05):42-44.
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HPLC法测定不同产地肉苁蓉中松果菊苷的含量()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
期数:
2009年05期
页码:
42-44
栏目:
技术与方法
出版日期:
1900-01-01

文章信息/Info

Title:
Determination of echinacoside in Herba Cistanchis from different regions by HPLC
作者:
刘雄 吴蓉 余晓晖 赵磊 季晓芳 李文萍
甘肃中医学院, 甘肃 兰州 730000
Author(s):
Liu Xiong Wu Rong Yu Xiaohui Zhao Lei Ji Xiaofang Li Wenping
Gansu College of TCM, Lanzhou 730000, China
关键词:
高效液相色谱法肉苁蓉松果菊苷含量测定
Keywords:
HPLCHerba Cistanchisechinacosidedetermination of the content
分类号:
R284.1
摘要:
目的 建立以高效液相色谱法测定不同产地肉苁蓉中松果菊苷含量的方法。方法 色谱柱为 Hypersil-ODS柱(4.6 mm×250 mm, 5μm);流动相:A相为1%醋酸溶液,B相为乙腈-甲醇(2:3);流速1.2 mL/min,检测波长334 nm,柱温30℃。结果 松果菊苷在0.22~2.2 μg范围内线性关系良好,r=0.9997,平均加样回收率为96.35%,RSD=2.03%。不同产地肉苁蓉中松果菊苷的含量不同(0.42%~1.52%)。结论 此法简便、准确、重现性好,可用于该药材的质量控制。
Abstract:
Objective To set up a method on determination of echinacoside in Herba Cistanchis from different regions by HPLC.Methods Chromatographic conditions were available as follows Hypersil-ODS column(4.6 mm×250 mm,5 μm),1% acetate solution as mobile phase A, acetonitrile and methanol(2:3) as mobile phase B, flowrate was 1.2 mL/min,and detecting wavelength was 334 nm, temperature of the column was 30℃.Results The linear correlation of echinacoside was well in 0.22~2.2 μg(r=0.9997), the average recovery rate was 96.35% with RSD of 2.03%.The contents of echinacoside in Herba Cistanchis from different regions were not the same(0.42%~1.52%).Conclusion The method is simple,accurate and has good reappearance.It can be used to control the quality of Herba Cistanchis.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2009-06-09;改回日期:2009-06-15。
作者简介:刘雄(1964-),男,教授,理学学士。研究方向:中药质量标准及化学成分的研究。
更新日期/Last Update: 1900-01-01