[1]陈玉武,张夙萍,高晓昱,等.党参药材中党参炔苷与浸出物含量的相关性研究[J].甘肃中医药大学学报,2010,(03):67-70.
 Chen Yuwu,Zhang Suping,Gao Xiaoyu,et al.Correlation study on the contents of lobetyolin and extracts in Codonopsis Pilosulae[J].Journal of Gansu University of Chinese Medicine,2010,(03):67-70.
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党参药材中党参炔苷与浸出物含量的相关性研究()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
期数:
2010年03期
页码:
67-70
栏目:
技术与方法
出版日期:
1900-01-01

文章信息/Info

Title:
Correlation study on the contents of lobetyolin and extracts in Codonopsis Pilosulae
作者:
陈玉武1 张夙萍1 高晓昱1 姚望录2 刘汉斌3
1. 定西市药品检验所, 甘肃 定西 743000;
2. 秦安县中医院, 甘肃 天水 743168;
3. 定西市第一人民医院, 甘肃 定西 743000
Author(s):
Chen Yuwu1 Zhang Suping1 Gao Xiaoyu1 Yao Wanglu2 Liu Hanbin3
1. Dingxi Institute for Drug Control, Dingxi 743000, China;
2. Qinan Hospital of TCM, Tianshui 743168, China;
3. First People’s Hospital in Dingxi, Dingxi 743000, China
关键词:
党参炔苷浸出物相关性HPLC法热浸法
Keywords:
lobetyolinextractscorrelationHPLC methodhot-maceration method
分类号:
R284.1
摘要:
目的 研究不同产地人工栽培党参药材中党参炔苷与浸出物的含量,对其测定结果进行比较,分析两者间量的相关性,为评价和控制党参药材质量提供参考依据。方法 采用HPLC法测定党参炔苷含量,色谱柱为Agilent TC-C18 柱(4.6 mm×250 mm,5 μm),以乙腈-水(30:70)为流动相,流速1.0 mL/min,检测波长267 nm,柱温30℃;以中国药典2005年版收载的热浸法,用45%乙醇作溶剂,测定党参浸出物(%)。结果 党参炔苷在0.19~0.96 μg/mL范围内线性关系良好,r=0.9997,精密度RSD为1.2%(n=5),重复性试验RSD为1.6%(n=6);平均回收率为99.4%,RSD为1.8%(n=6)。按药典标准判定,党参浸出物的含量均符合规定。结论 党参样品中党参炔苷与浸出物含量不成正比关系,说明党参药材中党参炔苷与浸出物间没有一定的内在相关性,这为科学评价和有效控制其质量提供了参考依据。
Abstract:
Objective To study the contents of lobetyolin and extracts in Codonopsis Pilosulae of artificial cultivation from different habitat, and compared the detecting results, analyzed the correlation of the contents between lobetyolin and extracts, provided reference basis for evaluating and controlling the quality of Codonopsis Pilosulae.Methods Using HPLC method to detect the content of lobetyolin. Chromatographic conditions were available as follows Agilent TC-C18 (4.6 mm× 250 mm,5 μm), acetonitrile and water (30:70) as mobile phase, flowrate was 1.0 mL/min, and detecting wavelength was 267 nm, temperature of the column was 30℃.The hot-maceration method that was recorded in Chinese Pharmacopoeia of 2005 edition was used to detect the extracts in Codonopsis Pilosulae, 45% alcohol as solvent.Results The linear correlation of lobetyolin was well in 0.19 to 0.96 μg(r=0.9997),RSD of precision was 1.2% (n=5),RSD of repetitive test was 1.6% (n=6), the average recovery rate was 99.4% with RSD of 1.8%(n=6).According the standard of pharmacopoeia to judge that the content of extracts in Codonopsis Pilosulae accorded with the stipulate.Conclusion The contents of lobetyolin and extracts in the sample of Codonopsis Pilosulae weren’t express the relationship of direct ratio, showed there wasn’t the inherent correlation between lobetyolin and extracts in Codonopsis Pilosulae.The result can provide reference basis for scientific evaluation and effective control of the quality of Codonopsis Pilosulae.

参考文献/References:

[1] 郑俊华.生药学[M].3版.北京:人民卫生出版社, 1987:184.
[2] 贺庆,朱恩圆,王峥涛.党参中党参炔苷HPLC分析[J].中国药学杂志,2005,40(1):56.
[3] 王润生,刘养清,郭继龙.山西党参药材中党参炔苷的含量分析[J].药物分析杂志,2007,27(8):1229.
[4] 彭锐,马鹏,李隆云.不同产地川党参药材的HPLC指纹图谱[J].中国中药杂志,2010,35(2):183.
[5] 刘养清,赵平,巫剑峰.党参饮片中党参内酯和党参炔苷的相关性研究[J].药物分析杂志,2008,28(1):1841.
[6] 中国药典委员会. 中华人民共和国药典(一部)[M].2005版.北京:化学工业出版社,2005:199,附录XA56.

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备注/Memo

备注/Memo:
收稿日期:2010-01-26;改回日期:2010-03-26。
作者简介:陈玉武(1969-),男,主管药师,主要从事药品质量检验工作。
更新日期/Last Update: 1900-01-01