[1]景明,罗永皎,陈正君,等.藏药湿生扁蕾药材反相高效液相层析指纹图谱的研究[J].甘肃中医药大学学报,2010,(06):18-21.
 Jing Ming,Luo Yongjiao,Chen Zhengjun,et al.Study of Tibetan Medicine Gentianopsis paludosa Ma on RP-HPLC fingerprint chromatogram[J].Journal of Gansu University of Chinese Medicine,2010,(06):18-21.
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藏药湿生扁蕾药材反相高效液相层析指纹图谱的研究()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
期数:
2010年06期
页码:
18-21
栏目:
中药研究
出版日期:
1900-01-01

文章信息/Info

Title:
Study of Tibetan Medicine Gentianopsis paludosa Ma on RP-HPLC fingerprint chromatogram
作者:
景明 罗永皎 陈正君 李硕
甘肃中医学院, 甘肃 兰州 730000
Author(s):
Jing Ming Luo Yongjiao Chen Zhengjun Li Shuo
Gansu College of TCM, Lanzhou 730000, China
关键词:
湿生扁蕾指纹图谱反相高效液相层析
Keywords:
Gentianopsis paludosa Mafingerprint chromatogramRP-HPLC
分类号:
R284.1
摘要:
目的 建立藏药湿生扁蕾药材反相高效液相色谱指纹图谱分析方法,为有效控制湿生扁蕾药材的质量奠定基础。方法 采用反相高效液相层析(RP-HPLC)技术,选用ZORBAX SB-C18色谱柱,甲醇-0.4%磷酸水溶液梯度洗脱,紫外检测波长为260 nm,流速为1.0 mL/min,记录时间为60 min,分析了10批湿生扁蕾药材的 HPLC指纹图谱。结果 在选定的色谱条件下通过相似度分析,确定10个色谱峰构成湿生扁蕾药材指纹图谱的特征峰。结论 采用RP-HPLC所建立的指纹图谱具有稳定、重复性好的特点,可用于湿生扁蕾药材的质量控制。
Abstract:
Objective To establish the analysis method of RP-HPLC fingerprint chromatogram of Tibetan Medicine Gentianopsis paludosa Ma,and lay a foundation for effective control of the quality of Gentianopsis paludosa Ma. Methods Adopted RP-HPLC technoloty,and selected ZORBAX SB-C18 chromatographic column,methanlo and water including 0.4% phosphoric acid as mobile phase,detecting wavelength was 260 nm,flowrate was 1.0 mL/min,the recording time was 60 minutes,The HPLC fingerprint chromatograms of 10 batches of Gentianopsis paludosa Ma were analysed. Results Through the analysis of similar degree under the selected chromatographic condition, 10 chromatographic peak was defined as the characteristic of forming fingerprint chromatogram of Gentianopsis paludosa Ma. Conlusion The fingerprint chromatogram adopting RP-HPLC to establish has steady and good repetitive characteristic,may be used to the quality control of Gentianopsis paludosa Ma.

参考文献/References:

[1] 张宝琛,甄润德,胡柏林,等.湿生扁蕾的化学成分研究[J].中草药,1980,11(4):149.
[2] 李作平,霍长虹,郑小莉.扁蕾的化学成分研究[J].中国现代应用药学杂志,2003,20(1):24.
[3] 王焕弟,谭成玉,白雪芳,等.藏药湿生扁蕾有效成分抑菌作用初探[J].天然产物研究与开发,2005,17(5):598.
[4] 国家食品药品监督管理局.关于印发《中药注射剂指纹图谱研究的技术要求(暂行)》的通知[J].中成药,2000,22(10):671-675.
[5] 谢培山.色谱指纹图谱分析是中草药质量控制的可行策略[J].中药新药与临床药理,2001,12(3):141-151.
[6] 任德权.中药指纹图谱质控技术的意义与应用[J].中药新药与临床药理,2001,12(3):135.

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备注/Memo

备注/Memo:
收稿日期:2010-07-07;改回日期:。
基金项目:甘肃省科技厅科技支撑计划项目(0804NKCA119-08)
作者简介:景明(1963-),男,教授,理学学士,主要从事中药新药的研究开发工作。
更新日期/Last Update: 1900-01-01