[1]李阳,赵磊,陈晖,等.高效液相色谱法测定舒康平喘胶囊中齐墩果酸的含量[J].甘肃中医药大学学报,2012,(04):58-60.
 Li Yang,Zhao Lei,Chen Hui,et al.Determination of the content of oleanolic acid in Shukang Pingchuan Capsule by HPLC[J].Journal of Gansu University of Chinese Medicine,2012,(04):58-60.
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高效液相色谱法测定舒康平喘胶囊中齐墩果酸的含量()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
期数:
2012年04期
页码:
58-60
栏目:
技术与方法
出版日期:
1900-01-01

文章信息/Info

Title:
Determination of the content of oleanolic acid in Shukang Pingchuan Capsule by HPLC
作者:
李阳 赵磊 陈晖 韩涛 余晓晖
甘肃中医学院, 甘肃省高校中(藏)药化学与质量研究省级重点实验室, 甘肃 兰州 730000
Author(s):
Li Yang Zhao Lei Chen Hui Han Tao Yu Xiaohui
Gansu College of Traditional Chinese Medicine, Provincial Key Laboratory of Traditional Chinese and Tibetan Medicine Chemistry and Quality Research of Universities in Gansu Province, Lanzhou 730000, China
关键词:
舒康平喘胶囊齐墩果酸高效液相色谱含量测定
Keywords:
Shukang Pingchuan Capsuleoleanolic acidHPLCcontent determination
分类号:
R284.2
摘要:
目的 建立舒康平喘胶囊中齐墩果酸的含量测定方法。方法 采用高效液相色谱(HPLC)法,色谱柱为AgiLent ZORBAX SB-C18(5.0 μm,4.6 mm×250 mm),柱温25℃,检测波长208 nm,流动相为乙腈-水(90:10),流速0.8 mL/min。结果 齐墩果酸在0.05~0.5 mg/mL范围内线性关系良好,r=0.9997(n=6),回归方程为Y=1.66×106X+1.4×104,平均回收率为96.21%,样品中齐墩果酸含量为1.82 mg/g。结论 该方法快速、准确、灵敏度高、重现性好,可作为舒康平喘胶囊中齐墩果酸的含量测定方法。
Abstract:
Objective To establish the method on determination of the content of oleanolic acid in Shukang Pingchuan Capsule.Methods Adopted HPLC method, chromatographic conditions were available as follows AgiLent ZORBAX SB-C18 column(5.0 μm,4.6 mm×250 mm),temperature of the column was 25℃,detecting wavelength was 208 nm, acetonitril and water (90:10) as mobile phase, the flowrate was 0.8 mL/min.Results The linear correlation of oleanolic acid was well within 0.05~0.5 mg/mL,r=0.9997 (n=6). The average recovery rate was 96.21%,the content of oleanolic acid in sample was 1.82 mg/g.Conclusion The method is quick, accurate, sensitive and reproducible,it can be used as the determination method of the content of oleanolic acid in Shukang Pingchuan Capsule.

参考文献/References:

[1] 韩涛,王佳蕾,韩澎,等.舒康平喘胶囊治疗支气管哮喘的药效学研究[J].中医研究,2008,21(3):12-15.
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[3] 谢乐,马晓黎,池玲玲,等.HPLC测定不同产地威灵仙中齐墩果酸的含量[J].中成药,2009,31(1):100-103.
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[6] 李显奇,何新荣,李晓蒙.HPLC测定女贞子不同炮制品中齐墩果酸的含量[J].广东药学院学报,1998,14(4):256-258.

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[1]王存琴,徐启祥,查淋芳,等.反相高效液相色谱法测定不同加工方法枸骨叶中熊果酸和齐墩果酸的含量[J].甘肃中医药大学学报,2014,31(02):21.
 Wang Cunqin,Xu Qixiang,Zha Linfang,et al.Quantitative analysis of ursolic acid and oleanolic acid in leaves of Ilex cornuta of different processing methods by RP-HPLC[J].Journal of Gansu University of Chinese Medicine,2014,31(04):21.

备注/Memo

备注/Memo:
收稿日期:2012-04-06;改回日期:2012-05-09。
作者简介:李阳(1977-),女,讲师,理学硕士。研究方向:中药化学。
更新日期/Last Update: 1900-01-01