[1]马维忠,李昶,常承东,等.反相高效液相色谱法测定藿香正气丸(浓缩丸)中橙皮苷的含量[J].甘肃中医药大学学报,2012,(06):16-19.
 Ma Weizhong,Li Chang,Chang Chengdong,et al.Determination of the content of Hesperidin in Huoxiang Zhengqi Pills(concentrated pill) by RP-HPLC[J].Journal of Gansu University of Chinese Medicine,2012,(06):16-19.
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反相高效液相色谱法测定藿香正气丸(浓缩丸)中橙皮苷的含量()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
期数:
2012年06期
页码:
16-19
栏目:
技术与方法
出版日期:
1900-01-01

文章信息/Info

Title:
Determination of the content of Hesperidin in Huoxiang Zhengqi Pills(concentrated pill) by RP-HPLC
作者:
马维忠1 李昶2 常承东3 肖吉元4
1. 甘肃奇正实业集团有限公司, 甘肃 兰州 730000;
2. 兰州太保药业有限公司, 甘肃 兰州 730050;
3. 甘肃中医学院附属医院药剂科, 甘肃 兰州 730020;
4. 兰州大学第二附属医院药剂科, 甘肃 兰州 730030
Author(s):
Ma Weizhong1 Li Chang2 Chang Chengdong3 Xiao Jiyuan4
1. Gansu Qizheng Industrial Group Limited Company,Lanzhou 730000, China;
2. Lanzhou Taibao Pharmaceutical Industry Limited Company, Lanzhou 730000, China;
3. Pharmacy Department, the Hospital Affiliated to Gansu College of TCM, Lanzhou 730020, China;
4. Pharmacy Department,the Second Hospital Affiliated to Lanzhou University, Lanzhou 730000, China
关键词:
反相高效液相色谱法藿香正气丸橙皮苷含量
Keywords:
RP-HPLC methodHuoxiang Zhengqi PillsHesperidincontent
分类号:
R284.2
摘要:
目的 建立藿香正气丸(浓缩丸)中橙皮苷的含量测定方法。方法 采用反相高效液相色谱(RP-HPLC)法。色谱条件:Phenomenex C18色谱柱,流动相为甲醇-水-冰醋酸(35:60:4),流速为1 mL/min,柱温40℃,检测波长283 nm。结果 橙皮苷在0.165 2~1.652 0 μg范围内呈良好的线性关系,回收率为98.55%,RSD=0.37%。结论 本法简便、准确,可以作为该制剂的质量控制方法。
Abstract:
Objective To establish the method on determination of the content of Hesperidin in Huoxiang Zhengqi Pills(concentrated pill). Methods Adopted RP-HPLC method, chromatographic conditions were available as follows Phenomenex C18 column,methanol-water-glacial acetic acid (35:60:4) as mobile phase, the flowrate was 1 mL/min,temperature of the column was 40℃,detecting wavelength was 283 nm. Results The linear correlation of Hesperidin was well within 0.165 2 to 1.652 0 μg.The average recovery rate was 98.55%, and RSD was 0.37%. Conclusion The method is simple and convenientquick and accurate, it can be used as the method of quality control of Huoxiang Zhengqi Pills(concentrated pill).

参考文献/References:

[1] 中华人民共和国卫生部药典委员会.中华人民共和国卫生部药品标准(中药成方制剂)[M].北京:人民卫生出版社,1993:205.
[2] 刘卫红.HPLC测定橙皮苷含量[J].中国实验方剂学杂志,2006,12(6):70-71.

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备注/Memo

备注/Memo:
收稿日期:2012-01-04;改回日期:2012-06-04。
作者简介:马维忠(1971-),男,工程师,医学学士,主要从事药品和保健食品的质量检验及质量管理工作。
通信作者:李昶(1969-),男,工程师,主要从事药品和保健食品的质量检验及质量管理工作。
更新日期/Last Update: 1900-01-01