[1]李阳,景明,韩涛.高效液相色谱法测定芩仙胶囊中黄芩苷含量[J].甘肃中医药大学学报,2013,(02):33-35.
 Li Yang,Jing Ming,Han Tao.Determination of baicalin in Qinxian Capsule by HPLC[J].Journal of Gansu University of Chinese Medicine,2013,(02):33-35.
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高效液相色谱法测定芩仙胶囊中黄芩苷含量()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
期数:
2013年02期
页码:
33-35
栏目:
技术与方法
出版日期:
2013-04-20

文章信息/Info

Title:
Determination of baicalin in Qinxian Capsule by HPLC
作者:
李阳12 景明12 韩涛12
1. 甘肃中医学院, 甘肃 兰州 730000;
2. 甘肃省高校中(藏)药化学与质量研究省级重点实验室, 甘肃 兰州 730000
Author(s):
Li Yang12 Jing Ming12 Han Tao12
1. Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China;
2. Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province, Lanzhou 730000, China
关键词:
芩仙胶囊黄芩苷高效液相色谱法含量测定
Keywords:
Qinxian CapsulebaicalinHPLCcontent determination
分类号:
R284.2
摘要:
目的 建立芩仙胶囊中黄芩苷的含量测定方法。方法 采用高效液相色谱法,色谱柱为ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5.0μm),柱温25℃,检测波长280 nm,流动相为甲醇-水-磷酸(47:53:0.2),流速1.0 mL/min。结果 黄芩苷在0.343 8~11.00 μg范围内线性关系良好,r=0.999 2(n=6),平均回收率为98.34%,样品中黄芩苷平均含量为70.34 mg/g。结论 该方法快速、准确、灵敏度高、重现性好,可用于芩仙胶囊的质量控制。
Abstract:
Objective To establish the determination method for the content of baicalin in Qinxian Capsule. Methods Using HPLC method,chromatographic column was ZORBAX Eclipse XDB-C18(4.6 mm×250 mm, 5.0 μm),column temperature was 25℃,detection wavelength was 280 nm, mobile phase was methanol-water-phosphoric acid(47:53:0.2),the flow rate was 1.0 mL/min. Results Baicalin showed a good linear relationship in range of 0.343 8~11.00 μg,r=0.999 2 (n=6).The average recovery was 98.34%.The average content of baicalin in sample was 70.34 mg/g. Conclusion The Method is quick,sensitive,accurate and reproducible.It can be used for the quality control of Qinxian Capsule.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-01-28
作者简介:李阳(1977-),女,讲师,理学硕士。研究方向:中药化学。
更新日期/Last Update: 1900-01-01