[1]王存琴,徐启祥,查淋芳,等.反相高效液相色谱法测定不同加工方法枸骨叶中熊果酸和齐墩果酸的含量[J].甘肃中医药大学学报,2014,31(02):21-23.
 Wang Cunqin,Xu Qixiang,Zha Linfang,et al.Quantitative analysis of ursolic acid and oleanolic acid in leaves of Ilex cornuta of different processing methods by RP-HPLC[J].Journal of Gansu University of Chinese Medicine,2014,31(02):21-23.
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反相高效液相色谱法测定不同加工方法枸骨叶中熊果酸和齐墩果酸的含量()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
31卷
期数:
2014年02期
页码:
21-23
栏目:
技术与方法
出版日期:
2014-05-25

文章信息/Info

Title:
Quantitative analysis of ursolic acid and oleanolic acid in leaves of Ilex cornuta of different processing methods by RP-HPLC
作者:
王存琴 徐启祥 查淋芳 苏军
皖南医学院药学院, 安徽 芜湖 241002
Author(s):
Wang Cunqin Xu Qixiang Zha Linfang Su Jun
College of Pharmacy, Wannan Medical College, Wuhu 241002, China
关键词:
枸骨叶熊果酸齐墩果酸反相高效液相色谱法
Keywords:
Ilex cornutaursolic acidoleanolic acidRP-HPLC
分类号:
R284.2
摘要:
目的 考察不同加工方法对枸骨叶中熊果酸和齐墩果酸含量的影响。方法 采用反相高效液相色谱法,梯度洗脱,对不同加工方法的枸骨叶甲醇提取物进行高效液相色谱分析。结果 熊果酸在55~550 μg/mL范围内呈良好的线性关系,r=0.999 9;齐墩果酸在35~350 μg/mL范围内呈良好的线性关系,r=0.999 9。晒干和阴干的样品中熊果酸和齐墩果酸含量较高,分别为1.45%,0.31%和1.43%,0.30%。加结论 该方法简便、重复性好、准确度高,为枸骨叶的合理加工提供了参考依据。
Abstract:
Objective To investigate the effects of different processing methods on ursolic acid and oleanolic acid contents in leaves of Ilex cornuta by using RP-HPLC method.Methods The methanol extract in leaves of Ilex cornuta of different processing methods was analysed by RP-HPLC method and gradient elution.Results The calibration curve of ursolic acid was linear in the range of 55~550 μg/mL, r=0.999 9 and the calibration curve of oleanolic acid was linear in the range of 35~350 μg/mL, r=0.999 9. The contents of ursolic acid and oleanolic acid in sun drying and shade-drying samples are higher. They were 1.45%, 0.31% and 1.43%, 0.30% respectively.Conclusion The established method is proved to be simple, reproductive and accurate. It provides the scientific basis for the rational processing of leaves of Ilex cornuta.

参考文献/References:

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相似文献/References:

[1]李国章,于华忠,曹庸.薄层扫描法测定三种中药材中熊果酸的含量[J].甘肃中医药大学学报,2006,(02):51.
 Liguozhang,Yuhuazhong,Cao Yong.Determination of the content of ursolic acid in three Traditional Chinese Medicines by the method of thin-layer chromatography[J].Journal of Gansu University of Chinese Medicine,2006,(02):51.

备注/Memo

备注/Memo:
收稿日期:2014-01-21
基金项目:安徽省高等学校省级自然科学研究重点项目(KJ2014A268);皖南医学院博士科研启动基金(201401)
作者简介:王存琴(1978-),女,讲师,理学博士,主要从事天然药物活性成分的研究与开发。E-mail:wcq5188b@163.com
更新日期/Last Update: 1900-01-01