[1]刘书斌,张樱山,李硕,等.甘肃不同产地党参中表征成分党参炔苷的含量分析[J].甘肃中医药大学学报,2014,31(05):15-19.
 Liu Shubin,Zhang Yingshan,Li Shuo,et al.Analysis on components of lobetyolin in Codonopsis pilosula from different planting areas in Gansu[J].Journal of Gansu University of Chinese Medicine,2014,31(05):15-19.
点击复制

甘肃不同产地党参中表征成分党参炔苷的含量分析()
分享到:

《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
31卷
期数:
2014年05期
页码:
15-19
栏目:
技术与方法
出版日期:
2014-10-25

文章信息/Info

Title:
Analysis on components of lobetyolin in Codonopsis pilosula from different planting areas in Gansu
作者:
刘书斌1 张樱山2 李硕1 高慧琴1 强正泽1 李成义1
1. 甘肃中医学院, 甘肃 兰州 730000;
2. 奇正藏药有限责任公司, 甘肃 兰州 730000
Author(s):
Liu Shubin1 Zhang Yingshan2 Li Shuo1 Gao Huiqin1 Qiang Zhengze1 Li Chengyi1
1. Gansu College of TCM, Lanzhou 730000, China;
2. Qizheng Tibetan Medicine Co. Ltd., Lanzhou 730000, China
关键词:
不同产地党参党参炔苷高效液相色谱含量测定
Keywords:
different planting areasCodonopsis pilosulalobetyolinHPLCcontent determination
分类号:
R284.2
摘要:
目的 比较甘肃不同产地党参中党参炔苷的含量差异。方法 采用高效液相色谱法测定不同产地党参中党参炔苷的含量,色谱条件为:ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5μL),流动相为乙腈-水(28:72),柱温30℃,检测波长269 nm。结果 以进样量(X)为横坐标,峰面积(Y)为纵坐标绘制标准曲线,得回归方程为Y=439.29X-610.08(R2=0.9992),说明党参炔苷含量在229.50~921.69μg/g范围内与峰面积呈良好的线性关系,其中渭源、临洮县产地的党参炔苷含量最高,漳县、宕昌县产地的含量最低。结论 不同产地党参的党参炔苷均有差异,其中甘肃渭源、临洮县主产地的党参表征成分含量较高,应为甘肃党参的主要适宜栽培区域。
Abstract:
Objective To compare the content difference of lobetyolin in Codonopsis pilosula planted in different areas of Gansu. Methods HPLC was used to determine the content of lobetyolin in Codonopsis pilosula from different areas. Chromatographic conditions were set as following: column (ZORBAX SB-C18[250 mm×4.6 mm, 5 μ L)], mobile phase [acetonitrile-water (28:72)], column temperature (30℃), detection wavelength (269 nm). Results With the amount of sample (X) as the abscissa, the peak area (Y) standard curves were plotted as the ordinate, the regression equation turned out to be Y=439.29X-610.08 (R2=0.999 2), indicating the content of lobetyolin falling in 229.50~921.69 μg/g scope showed a good linear relationship with the peak area. The contents of lobetyolin in Codonopsis pilosula planted in Weiyuan and Lintao were higher than of other places, of which from Zhangxian and Tanchang were lowest. Conclusion Contents of lobetyolin in Codonopsis pilosula from various planting areas in Gansu are significantly different. Weiyuan and Lintao can be specified as the main planting areas for producing Codonopsis pilosula.

参考文献/References:

[1] 国家药典委员会.中国药典2010年版(一部)[M].北京:化学工业出版社,2010:264.
[2] 蒋小飞,张宝顺.党参总皂苷的提取分离和纯化[J].安徽农业科学,2011,39(4):2061-2063.
[3] 杨亚蕾.党参炔苷与浸出物含量的相关性研究[J].中国中医药咨讯,2010,33(2):14-16.
[4] 杨耀强,李成义,魏学明,等.不同商品等级党参的鉴定学研究[J].甘肃中医学院学报,2011,28(4):61-63.
[5] 时军,马方励,廖华卫,等.HPLC 法测定不同产地党参药材中党参炔苷的含量[J].广东药学院学报,2011,27(1):51-53.
[6] 宋丹,程雪梅,李隆云,等.HPLC测定不同产地川党参中党参炔苷的含量[J].中国中药杂志,2008,33(17):2133-2135.
[7] 彭锐,马鹏,李隆云,等.不同产地川党参药材的HPLC指纹图谱[J].中国中药杂志,2010,35(2):183-186.
[8] 王润生,刘养清,郭继龙,等.山西党参中党参炔苷含量分析[J].药物分析杂志,2007,27(28):1229-1231.
[9] 刘养清,赵平,巫剑峰,等.党参饮片中党参内酯和党参炔苷的相关性研究[J].药物分析杂志,2008,28(11):1841-1844.
[10] 冯月,吴文夫,魏建华,等.正交设计优化党参炔苷提取工艺的研究[J].人参研究,2013(2):46-48.
[11] 王玉明,李锦,张丽媛.药用植物次生代谢产物积累规律的研究概况[J].中南药学,2012,10(2):136-138.
[12] 彭锐,孙年喜,马鹏,等.川党参品质形成及影响因素研究[J].时珍国医国药,2010,21(4):864-865.
[13] 彭锐,王远会,马鹏.黄花蒿中青蒿素含量与产地气象因子及土壤的关系研究[J].西南农业学报,2010,16(2):519-522.

相似文献/References:

[1]白娟,邱桐,李萍,等.党参治疗呼吸窘迫综合征的实验研究——血气和酸碱的变化[J].甘肃中医药大学学报,1993,(03):49.
 Bai Juan,Qiu Tong,Li Ping,et al.A Laboratry Study of Codonopsis on changes of Qi and Blood and Acid-base in RDS of rats[J].Journal of Gansu University of Chinese Medicine,1993,(05):49.
[2]白娟,邱桐,李萍.党参治疗呼吸窘迫综合征的实验研究-肺泡-动脉血氧分压差和气-血屏障的变化[J].甘肃中医药大学学报,1994,(01):50.
 Bai Juan,Qiu Tong,Li Ping.Effects of Codonopsis on Changes of Alveolar-arterial Oxygen Gradient and Blood-air Barrier in Rats with Respiratory Distress Syndrome[J].Journal of Gansu University of Chinese Medicine,1994,(05):50.
[3]杨耀强,李成义,魏学明,等.不同商品等级党参的鉴定学研究[J].甘肃中医药大学学报,2011,(04):61.
[4]马琴国,赵勇,安培坤,等.党参与素花党参花粉形态扫描电镜观察[J].甘肃中医药大学学报,2010,(03):71.
[5]崔治家,李富云,候嘉,等.甘肃不同产地黄芪芒柄花素的含量测定[J].甘肃中医药大学学报,2012,(01):56.
 Cui Zhijia,Li Fuyun,Hou Jia,et al.Determination of the content of formononetin in Astragalus from different habitats in Gansu[J].Journal of Gansu University of Chinese Medicine,2012,(05):56.
[6]王惠珍,张恩和,马啸,等.党参根系分泌物对自身种子萌发的影响[J].甘肃中医药大学学报,2007,(06):36.
 Wang Huizhen,Zhang enhe,Ma Xiao,et al.Effects of root exudates of Radix Codonopsis Pilosula on self-seeds germination[J].Journal of Gansu University of Chinese Medicine,2007,(05):36.
[7]张延红,何春雨,林丽,等.赤霉素对甘肃道地党参种子萌发的影响[J].甘肃中医药大学学报,2008,(06):10.
 Zhang Yanhong,He Chunyu,Lin li,et al.Effect of GA3 on germination of genuine Gansu Codonopsis pilosula (Franch.) Nannf. Seeds[J].Journal of Gansu University of Chinese Medicine,2008,(05):10.
[8]王惠珍,杜弢,陆国弟,等.硅提高干旱胁迫下党参种子萌发潜力研究[J].甘肃中医药大学学报,2015,32(01):30.
 WANG Huizhen,DU Tao,LU Guodi,et al.Study on silicon in promoting seed germination potential of Codonopsis pilosula under drought stress[J].Journal of Gansu University of Chinese Medicine,2015,32(05):30.
[9]白娟,邱桐,李萍,等.党参治疗呼吸窘迫综合征实验研究——肺表面活性物质含量及板层小体的变化[J].甘肃中医药大学学报,1997,(03):22.
[10]李丹明,李红芳,李伟,等.党参和丹参对兔离体主动脉平滑肌运动的影响[J].甘肃中医药大学学报,2000,(02):15.
 Li Dan-ming.Effects of Dangshen and Danshen on contractile activity of isolated aortic muscle strips in abbits[J].Journal of Gansu University of Chinese Medicine,2000,(05):15.

备注/Memo

备注/Memo:
收稿日期:2014-04-09
基金项目:甘肃省中药材产业科技攻关项目(GYC13-06)
作者简介:刘书斌(1988-),男,在读硕士研究生。研究方向:中药资源与质量综合评价。
通信作者:李成义(1964-),男,教授,博士生导师。研究方向:中药品种与质量研究。Tel:(0931)8765385, E-mail:gslichengyi@163.com
更新日期/Last Update: 1900-01-01