[1]顾崇梅,王海涛,祁玉宁.安神解郁胶囊质量控制方法的研究[J].甘肃中医药大学学报,2014,31(05):28-31.
 Gu Chongmei,Wang Haitao,Qi Yuning.Research of quality control standard for Anshenjieyu Capsule[J].Journal of Gansu University of Chinese Medicine,2014,31(05):28-31.
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安神解郁胶囊质量控制方法的研究()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
31卷
期数:
2014年05期
页码:
28-31
栏目:
技术与方法
出版日期:
2014-10-25

文章信息/Info

Title:
Research of quality control standard for Anshenjieyu Capsule
作者:
顾崇梅1 王海涛2 祁玉宁1
1. 酒泉市药品检验检测中心, 甘肃 酒泉 735000;
2. 酒泉市中医院, 甘肃 酒泉 735000
Author(s):
Gu Chongmei1 Wang Haitao2 Qi Yuning1
1. Jiuquan Drug Inspection Center, Jiuquan 735000, China;
2. Jiuquan TCM Hospital, Jiuquan 735000, China
关键词:
安神解郁胶囊质量标准薄层色谱法高效液相色谱法
Keywords:
Anshenjieyu Capsulequality control standardTLCHPLC
分类号:
R284.1
摘要:
目的 建立安神解郁胶囊定性、定量检测方法。方法 采用薄层色谱(TLC)法鉴别方中酸枣仁、丹参、黄连、石菖蒲,采用高效液相色谱(HPLC)法测定酸枣仁皂苷A和B的含量。色谱条件:色谱柱为Inertsil ODS-SP(4.6 mm×250 mm,5μm),流动相为乙腈-水(65:35),流速为1.0 mL/min,柱温30℃,漂移管温度为40℃,空气压力为340 kPa,检测器为蒸发光检测器。结果 定性鉴别薄层色谱特征明显;HPLC法测得酸枣仁皂苷A和B的线性范围分别为0.516~10.320μg/mL(r=0.9996)、0.248~4.970μg/mL(r=0.9993),平均加样回收率(n=6)分别为97.40%(RSD=1.20%)、95.97%(RSD=1.70%)。结论 TLC方法操作简单,结果可靠,重现性好,可有效地控制该制剂的质量。
Abstract:
Objective To establish the qualitative and quantitative methods for Anshenjieyu Capsule. Methods Ziziphi Spinosa Semen, Salviae Miltiorrhizae Radix et Rhizoma, Coptidis Rhizoma and Acori Tatarinowii Rhizoma were identified by TLC. The content of Jujuboside A and Jujuboside B were determined by HPLC. HPLC method was performed on a Inertsil ODS-SP (4.6 mm×250 mm, 5 μm) with the mobile phase of acetonitrile-water (65:35) and flow rate of 1.0 mL/min. The column temperature was 30℃. The temperature of drift tube was 40℃. The air pressure was 340 kPa and evaporative light detector was used. Results Characteristics of qualitation identification were obvious. The linear ranges of Jujuboside A and Jujuboside B were 0.516~10.320 μg/mL(r=0.999 6) and 248~4.970 μg/mL(r=0.999 3). The average recoveries (n=6) were 97.40% (RSD=1.20%) and 95.97% (RSD=1.70%) respectively. Conclusion The method is good reproducibility. It is accurate and suitable in controlling quality of Anshenjieyu Capsule availably.

参考文献/References:

[1] 国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2010:70,85,285,290,343.
[2] 王燕,柴程芝,朱丹妮.HPLC-ELSD测定GSSM颗粒中酸枣仁皂苷A和人参皂苷Rb1[J].中国实验方剂学杂志,2011,17(12):76-78.
[3] 闫艳,杜晨晖,李小菊,等. HPLC-DAD-ELSD法同时测定酸枣仁中斯皮诺素、酸枣仁皂苷A和B的含量[J].药物分析杂志, 2011,31(1):30-33.
[4] 曹磊,[日]瑞裕树.中药材高效液相色谱检定:《中华人民共和国药典》(2010年版)收载品种对应方法集[M].北京:化学工业出版社,2013:699-700.

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备注/Memo

备注/Memo:
收稿日期:2014-03-03
作者简介:顾崇梅(1973-),女,副主任药师,理学学士,主要从事药品检验工作。
更新日期/Last Update: 1900-01-01