[1]马丹,郭玫,朱冉,等.高效液相色谱内标法测定当归滴丸中藁本内酯的含量[J].甘肃中医药大学学报,2014,31(06):29-31.
 Ma Dan,Guo Mei,Zhu Ran,et al.Determination of ligustilide in Danggui Dropping Pill by HPLC internal standard method[J].Journal of Gansu University of Chinese Medicine,2014,31(06):29-31.
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高效液相色谱内标法测定当归滴丸中藁本内酯的含量()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
31卷
期数:
2014年06期
页码:
29-31
栏目:
技术与方法
出版日期:
2014-12-25

文章信息/Info

Title:
Determination of ligustilide in Danggui Dropping Pill by HPLC internal standard method
作者:
马丹1 郭玫12 朱冉1 魏舒畅1
1. 甘肃中医学院药学系, 甘肃 兰州 730000;
2. 甘肃省高校中(藏)药化学与质量省级重点实验室, 甘肃 兰州 730000
Author(s):
Ma Dan1 Guo Mei12 Zhu Ran1 Wei Shuchang1
1. Pharmacy Department in Gansu College of TCM, Lanzhou 730000, China;
2. Gansu Provincial Key Laboratory for Chinese Tibet Herbal Chemicals and Quality Research, Lanzhou 730000, China
关键词:
当归滴丸藁本内酯补骨脂素高效液相色谱内标法含量测定
Keywords:
Danggui Dropping PillligustilidepsoralenHPLC internal standard methodassaying
分类号:
R284.2
摘要:
目的 建立当归滴丸中藁本内酯的含量测定方法。方法 采用高效液相色谱法测定当归滴丸中藁本内酯的含量,色谱柱为Thermo ODS-C18(4.6 mm×250 mm,5 μm),流动相为甲醇:水(冰乙酸调pH至4)=55:45,流速为1 mL/min,检测波长325 nm,柱温25℃。 结果 藁本内酯在39.58~395.80 μg/mL范围内呈良好的线性关系,回归方程为Y=0.0316X-0.1482,r=0.9996,平均回收率为101.50%,藁本内酯含量为0.524%。结论 该方法准确、简便、可行、精密度高、重复性良好,可作为当归滴丸中藁本内酯的定量测定方法。
Abstract:
Objective To establish a method for determining the content of ligustilide in Danggui Dropping Pill by HPLC internal standard method. Methods The column was Thermo ODS-C18(4.6 mm×250 mm, 5 μm), the mobile phase consisted of methanol-acetic acid:water (pH=4) (55:45), the flow rate was 1 mL/min, and UV detection wave length was set at 325 nm as well as the temperature was set at 25℃. Results Ligustilide showed a good linearity in the rang of 39.58~395.80 μg/mL (r=0.9996). The regression equation was Y=0.0316X-0.1482, r=0.9996 and the average recovery rate was 101.5%. The content of ligustilide was 0.524%. Conclusion The method is simple, accurate and precise. It can meet the requirements of quality control of ligustilide in the pills.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2014-03-17
基金项目:兰州科技计划项目(2010-1-160)。
作者简介:马丹(1988-),女,在读硕士研究生。研究方向:中药有效成分及质量标准研究。
通信作者:郭玫(1963-),女,教授,理学硕士。研究方向:中药有效成分与质量标准研究。Tel:0931-8765394,E-mail:liyingjun59520@sina.com
更新日期/Last Update: 1900-01-01