[1]李成明,刘效栓,李喜香,等.高效液相色谱法测定慢支胶囊中柚皮苷的含量[J].甘肃中医药大学学报,2015,32(02):24-27.
 LI Chengming,LIU Xiaoshuan,LI Xixiang,et al.Determination of naringin in Manzhi Capsule by HPLC[J].Journal of Gansu University of Chinese Medicine,2015,32(02):24-27.
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高效液相色谱法测定慢支胶囊中柚皮苷的含量()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
32卷
期数:
2015年02期
页码:
24-27
栏目:
技术与方法
出版日期:
2015-04-25

文章信息/Info

Title:
Determination of naringin in Manzhi Capsule by HPLC
作者:
李成明1 刘效栓1 李喜香1 石文2
1. 甘肃省中医院药剂科, 甘肃 兰州 730050;
2. 甘肃省食品药品检验所, 甘肃 兰州 730070
Author(s):
LI Chengming1 LIU Xiaoshuan1 LI Xixiang1 SHI Wen2
1. Pharmacy of Gansu TCM Hospital, Lanzhou 730050, China;
2. Gansu Food and Drug Inspective Office, Lanzhou 730070, China
关键词:
慢支胶囊柚皮苷高效液相色谱法含量测定
Keywords:
Manzhi CapsulenaringinHPLCdetermination of the content
分类号:
R284.2
摘要:
目的 制定慢支胶囊中柚皮苷的含量测量方法。方法 采用高效液相色谱(HPLC)法测定慢支胶囊中柚皮苷的含量,色谱条件为:C18色谱柱(4.6 mm×150 mm),流动相为甲醇-水(40∶60),检测波长为283 nm。并对准确度、精密度、专属性等进行方法学考察。结果 柚皮苷在0.1952~1.9520 μg范围内与峰面积呈良好的线性关系,回归方程为Y=6.466×106X+8.342×103,r=0.9999,平均回收率为98.38%,柚皮苷含量为15.05 mg/g。结论 该方法灵敏、准确、重现性好、精密度高、专属性强,可作为慢支胶囊中柚皮苷的定量测定方法。
Abstract:
Objective To establish a method for determining naringin in Manzhi Capsule. Methods The C18 column (4.6 mm×150 mm) was used. The mobile phase was methanol-water (40∶60). The detection wavelength was set at 283 nm. Accuracy, precision and specificity were studied based on methodology. Results The linear range of naringin was within 0.1952~1.9520 μg. The regression equation was Y=6.466×106X+8.342×103, r=0.9999, the average recovery rate was 98.38% and the content of naringin was 15.05 mg/g. Conclusion The method is sensitive, accurate and of good reproducibility, precision and specificity. It can be used to determine the naringin in Manzhi Capsule.

参考文献/References:

[1] 国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2010:69-70.
[2] 尚明三,李振国.现代实用中药质量控制技术[M].北京:人民卫生出版社,2003:312-315.
[3] 丛旭东.复方咳喘宁胶囊质量标准研究[J].河北医药,2007,29(4):413-416.
[4] 涂文升,黄其春.HPLC测定心通口服液中柚皮苷的含量[J].中国药师,2009,12(3):1840-1843.
[5] 黄海卫.HPLC法测定胃痛丸中柚皮苷的含量[J].中医药导报,2009,15(6):81-84.

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 LI Caidong,PAN Xinbo,WANG Xin,et al.Study on the content determination and preliminary stability of Jinyin Lidan Oral Liquid[J].Journal of Gansu University of Chinese Medicine,2015,32(02):29.

备注/Memo

备注/Memo:
收稿日期:2014-4-22
作者简介:李成明(1978-),男,药师,医学硕士,主要从事中药有效成分与质量标准研究。
更新日期/Last Update: 1900-01-01