[1]赵小梅,杨志军,袁文珺,等.正交试验优选苦柏洗剂提取工艺研究[J].甘肃中医药大学学报,2016,33(04):40-43.[doi:10.16841/j.issn1003-8450.2016.04.12]
 ZHAO Xiaomei,YANG Zhijun,YUAN Wenjun,et al.Study on the optimization of the extraction technology for Kubai Lotion by orthogonal experiment[J].Journal of Gansu University of Chinese Medicine,2016,33(04):40-43.[doi:10.16841/j.issn1003-8450.2016.04.12]
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正交试验优选苦柏洗剂提取工艺研究()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
33卷
期数:
2016年04期
页码:
40-43
栏目:
工艺研究
出版日期:
2016-08-25

文章信息/Info

Title:
Study on the optimization of the extraction technology for Kubai Lotion by orthogonal experiment
作者:
赵小梅 杨志军 袁文珺 吴晶
兰州市中医医院药剂科, 甘肃 兰州 730050
Author(s):
ZHAO Xiaomei YANG Zhijun YUAN Wenjun WU Jing
Pharmacy of Lanzhou TCM Hospital, Lanzhou, Gansu, 730050, China
关键词:
苦柏洗剂小檗碱含量测定提取工艺正交试验
Keywords:
Kubai Lotionberberinedeterminationextraction technologyorthogonal experiment
分类号:
R283.6
DOI:
10.16841/j.issn1003-8450.2016.04.12
摘要:
目的 测定苦柏洗剂中盐酸小檗碱的含量,优化苦柏洗剂的提取工艺。方法 采用高效液相色谱(HPLC)法对苦柏洗剂中盐酸小檗碱的含量进行测定,色谱柱为C18(150 mm×4.6 mm,5 μm),以乙腈-0.1%磷酸水溶液(0 min,20%乙腈;10 min,40%乙腈)为流动相,流速1.0 mL/min,进样量10 μL,检测波长265 nm,柱温30℃;采用正交试验对苦柏洗剂的提取工艺进行优化。结果 盐酸小檗碱的线性回归方程为Y=168 427X-22 546,r=0.999 9,盐酸小檗碱在16.9~84.5 μg/mL范围内呈良好线性关系。加样回收率平均值为98.44%,RSD值为1.43%。正交试验结果为加20倍量水,提取2次,每次1.5 h。结论 本试验结果为苦柏洗剂的开发提供了实验理论依据,该工艺稳定、可靠,可应用于工业化大量生产。
Abstract:
Objective To optimize the extraction technology for Kubai Lotion by determining the content of berberine hydrochloride. Methods The content of berberine hydrochloride in Kubai Lotion was determined by HPLC. The sample was separated on the column of C18(150 mm×4.6 mm, 5 μm) which moving phase was acetonitrile and 0.1% phosphoric acid solution (0 min, 20% acetonitrile; 10 min, 40% acetonitrile). The flow rate was 1.0 mL/min; the sample size was 10 μL; the UV detection wavelength was 265 nm; the column temperature was 30℃. The extraction technology for Kubai Lotion was optimized by orthogonal experiment. Results The liner equation for berberine hydrochloride was Y=168 427X-22 546, r=0.999 9; the calibration curves for berberine hydrochloride was in good linearity in the range of 16.9~84.5 μg/mL; the average recovery was 98.44% with RSD of 1.43%. The orthogonal experiment result was:adding 20 times water, extracting for 2 times and each time for 1.5 h. Conclusion The study provides experimental basis for the exploitation of Kubai Lotion. The method is stable and reliable, can be used for large-scale industrialized production.

参考文献/References:

[1] 国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2010:188.
[2] 谭元生,胡宏,黄大香,等.复方苦参洗液的药效学研究[J].中药药理与临床,1999,15(2):36-38.
[3] 王鲁萍,宋磊,黄桢.苦参碱对鼠实验性皮炎的抗炎止痒作用[J].世界感染杂志,2005,5(6):471-474.
[4] 陈凌,骆凯,吴斌贝,等.氧化苦参碱抗炎作用研究新进展[J].医学综述,2007,13(15):1167-1169.
[5] 高红旺,张文希,田济民,等.不同工艺对黄柏中黄连素提取量的影响[J].第四军医大学吉林军医学院学报,2003,25(1):29.

备注/Memo

备注/Memo:
收稿日期:2016-03-05
作者简介:赵小梅(1969-),女,副主任中药师,理学学士。研究方向:医院药学管理与中药鉴定。
通信作者:杨志军(1968-),男,副主任药师,医学学士。研究方向:医院药学与医院院内制剂。E-mail:809756156@qq.com
更新日期/Last Update: 1900-01-01