[1]冯华,刘茂永,刘英波,等.苗药夜关门药材质量标准研究[J].甘肃中医药大学学报,2017,34(03):23-27.[doi:10.16841/j.issn1003-8450.2017.03.07]
 FENG Hua,LIU Maoyong,LIU Yingbo,et al.Study on the quality standard of Lespedeza cuncata (Dum.Cours.) G. Don.[J].Journal of Gansu University of Chinese Medicine,2017,34(03):23-27.[doi:10.16841/j.issn1003-8450.2017.03.07]
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苗药夜关门药材质量标准研究()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
34卷
期数:
2017年03期
页码:
23-27
栏目:
技术与方法
出版日期:
2017-06-25

文章信息/Info

Title:
Study on the quality standard of Lespedeza cuncata (Dum.Cours.) G. Don.
作者:
冯华1 刘茂永2 刘英波3 刘亮3 潘年松3
1. 遵义市食品药品检验所中药室, 贵州 遵义 563002;
2. 遵义市第一人民医院妇产科, 贵州 遵义 563002;
3. 遵义医药高等专科学校药学系, 贵州 遵义 563002
Author(s):
FENG Hua1 LIU Maoyong2 LIU Yingbo3 LIU Liang3 PAN Niansong3
1. TCM Office of Zunyi Institute for Food and Drug Control, Zunyi, Guizhou, 563002, China;
2. Obstetrics and Gynecology Department, the First People’s Hospital in Zunyi City, Zunyi, Guizhou, 563002, China;
3. Pharmacy School, Zunyi Medical and Pharmaceutical College, Zunyi, Guizhou, 563002, China
关键词:
苗药夜关门槲皮素薄层色谱高效液相色谱含量测定
Keywords:
Miao national herbsLespedeza cuncata (Dum. Cours.) G. Don.quercetinTLCHPLCcontent determination
分类号:
R284.2
DOI:
10.16841/j.issn1003-8450.2017.03.07
摘要:
目的 建立苗药夜关门药材的定性、定量检测方法,并制定质量标准。方法 对苗药夜关门药材进行性状鉴别、显微鉴别,采用薄层色谱(TLC)法对其中的槲皮素进行定性鉴别,采用高效液相色谱(HPLC)法对其中的槲皮素进行含量测定;参照《中华人民共和国药典》2015版一部附录方法对苗药夜关门药材的水分、总灰分、醇溶性浸出物含量进行测定。结果 薄层色谱斑点清晰,专属性强,且阴性无干扰;含量测定中,槲皮素的回归方程为Y=1 114.5X+43.507 2,r=0.999 9,结果表明槲皮素在0.128~1.280 μg范围内线性关系良好;平均回收率为100.37%;初步预定苗药夜关门药材水分不得超过10.0%,总灰分不得超过6.0%,醇溶性浸出物含量不得少于12.0%。结论 本法操作简单、结果可靠,可有效控制苗药夜关门药材的质量。
Abstract:
Objective To establish qualitative and quantitative test methods for Lespedeza cuncata (Dum. Cours.) G. Don. (Miao national herb) and work out the quality standard. Methods Morphological identification and microscopic identification of Lespedeza cuncata (Dum. Cours.) G. Don. were made. Quercetin in it was qualitatively identified by TLC. Content of quercitrin was determined by HPLC. The water content,total ashes,ethanol-soluble extractives were determined according to the approaches listed in Chinese Pharmacopeoa published in 2015. Results The TLC spots were clear without interference and had a strong specificity. The content of quercetin was in good line with the range of 0.128 to 1.280 μg,Y=1 114.5X+43.507 2,r=0.999 9. The average recovery was 100.37%. It was initially proposed that the content of moisture was at most 10.0%,total ash was no more than 6.0%,ethanol-soluble extractives was at most 12.0%. Conclusion The method is simple,reliable and can effectively control the quality of medicinal materials.

参考文献/References:

[1] 林亚平,叶阳明,林开中,等.贵州省中药材、民族药材质量标准[M].贵阳:贵州科技出版社,2003:257.
[2] 王国强,黄璐琦,郝近大,等.全国中草药汇编(第2卷)[M].3版.北京:人民卫生出版社,2014:674.
[3] 国家药典委员会.中华人民共和国药典(四部)[M].北京:中国医药科技出版社,2015:103.
[4] 马雯芳,谢凤凤,曾锦燕,等.瑶药倒丁风质量标准初步研究[J].时珍国医国药,2014,25(10):2420-2421.
[5] 吴林菁,聂明华,冯华,等.酢浆草显微鉴别的专属性研究[J].中国实验方剂学杂志,2013,19(2):133.
[6] 冯华,石尚友,聂明华,等.土知母药材中射干苷定性与定量分析研究[J].世界科学技术-中医药现代化,2014,16(1):78-80.
[7] 邹孔强,聂明华,罗秀琼,等.连朴饮不同煎液中栀子苷的薄层色谱鉴别[J].国际中医中药杂志,2012,34(4):330-331.
[8] 梁远园,黄有带,熊艺花,等.妇血安薄膜衣片的质量标准[J].中国医药工业杂志,2013,44(5):487-491.
[9] 吕彬.清热解毒口服液薄层鉴别的改进[J].中成药,2007,29(10):1560.

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备注/Memo

备注/Memo:
收稿日期:2016-05-09。
基金项目:贵州省高层次创新型人才培养项目(遵市科合人才〔2015〕35号);贵州省科技合作计划项目(黔科合LH字〔2015〕7580号)。
作者简介:冯华(1977-),男,副主任药师,理学硕士,主要从事药品检验及新药研究工作。
通信作者:潘年松(1966-),男,教授,硕士生导师,理学博士,主要从事药理急性毒性的研究工作。Tel:13765963366
更新日期/Last Update: 1900-01-01