[1]陈正君,卢年华,赵慧巧,等.反相高效液相色谱法测定复方湿生扁蕾结肠靶向微丸的体外释放度[J].甘肃中医药大学学报,2017,34(04):19-23.[doi:10.16841/j.issn1003-8450.2017.04.07]
 CHEN Zhengjun,LU Nianhua,ZHAO Huiqiao,et al.Evaluation of release degree in vitro of compound Gentianopsis paludosa colon-targeting pellets by RP-HPLC[J].Journal of Gansu University of Chinese Medicine,2017,34(04):19-23.[doi:10.16841/j.issn1003-8450.2017.04.07]
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反相高效液相色谱法测定复方湿生扁蕾结肠靶向微丸的体外释放度()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
34卷
期数:
2017年04期
页码:
19-23
栏目:
技术与方法
出版日期:
2017-08-25

文章信息/Info

Title:
Evaluation of release degree in vitro of compound Gentianopsis paludosa colon-targeting pellets by RP-HPLC
作者:
陈正君1 卢年华2 赵慧巧2 芦彦兆2 陈晖2 景明2
1. 甘肃中医药大学期刊编辑部, 甘肃 兰州 730000;
2. 甘肃中医药大学药学院, 甘肃 兰州 730000
Author(s):
CHEN Zhengjun1 LU Nianhua2 ZHAO Huiqiao2 LU Yanzhao2 CHEN Hui2 JING Ming2
1. Journal Editorial Department, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China;
2. Pharmacy College, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China
关键词:
复方湿生扁蕾结肠靶向微丸释放度反相高效液相色谱
Keywords:
Gentianopsis paludosacolon-targeting pelletsrelease rateRP-HPLC
分类号:
R284.2
DOI:
10.16841/j.issn1003-8450.2017.04.07
摘要:
目的 对制取的具pH依赖型和酶触依赖型的微丸进行模拟体内环境试验,以判断制备复方湿生扁蕾结肠靶向微丸的可行性。方法 结合反相高效液相色谱(RP-HPLC)法,以湿生扁蕾中所含木犀草素为评价指标,结合2015版《中华人民共和国药典》中释放度测定法,评价微丸在模拟胃液、小肠液、结肠液中的释药行为。结果 复方湿生扁蕾微丸中木犀草素在模拟胃液和小肠液中2 h后均未见释放;在模拟结肠液中1 h后释放度为85.3%,2 h后达95.3%。结论 制备的复方湿生扁蕾结肠靶向微丸释放度满足《中华人民共和国药典》要求,工艺可靠,重现好,能达到预期释药效果。
Abstract:
Objective To determine the preparation feasibility of Gentianopsis paludosa colon-targeting pellets by simulating environment in vivo for the pH-dependent and enzyme-dependent pellets which are prepared. Methods According to the release measurement recorded in Chinese pharmacopoeia (2015), the content of luteolin was taken as the evaluation index to determine the release degree of the pellets in simulated conditions of gastric, intestinal and colonic juice with the help of RP-HPLC. Results Luteolin was neither released in simulated gastric juice, nor in intestinal juice in 2 hours. But its release rate was 85.3% in colonic juice in 1 hour and even reached 95.3% in 2 hours. Conclusion The dissolution rate of prepared colon-targeting pellets is in line with the measurement in Chinese pharmacopoeia. The preparation technology is reliable and reproducible which gets the desired release effect.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-08-20。
基金项目:国家自然科学基金地区基金项目(81560717)。
作者简介:陈正君(1986-),男,讲师,医学硕士。研究方向:中藏药新制剂开发研究及期刊编辑工作。
通信作者:景明(1962-),男,教授,博士生导师,主要从事中药新剂型、新制剂的研究。E-mail:1339512509@qq.com
更新日期/Last Update: 1900-01-01