[1]冯华,石尚友,罗秀琼,等.苗药复方胃痛胶囊中吴茱萸的定性与定量分析[J].甘肃中医药大学学报,2017,34(04):30-34.[doi:10.16841/j.issn1003-8450.2017.04.09]
 FENG Hua,SHI Shangyou,LUO Xiuqiong,et al.Qualitative and quantitative analysis on evodiamine in Fufang Weitong Jiaonang[J].Journal of Gansu University of Chinese Medicine,2017,34(04):30-34.[doi:10.16841/j.issn1003-8450.2017.04.09]
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苗药复方胃痛胶囊中吴茱萸的定性与定量分析()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
34卷
期数:
2017年04期
页码:
30-34
栏目:
技术与方法
出版日期:
2017-08-25

文章信息/Info

Title:
Qualitative and quantitative analysis on evodiamine in Fufang Weitong Jiaonang
作者:
冯华1 石尚友1 罗秀琼1 王世俊1 郑凰雅1 王样培2
1. 遵义市食品药品检验所中药室, 贵州 遵义 563002;
2. 贵阳中医学院药学院, 贵州 贵阳 550002
Author(s):
FENG Hua1 SHI Shangyou1 LUO Xiuqiong1 WANG Shijun1 ZHENG Huangya1 WANG Yangpei2
1. TCM Office, Zunyi Institute for Food & Drug Control, Zunyi, Guizhou, 563002, China;
2. Pharmacy College, Guiyang College of Traditional Chinese Medicine, Guiyang, Guizhou, 550002, China
关键词:
苗药复方胃痛胶囊吴茱萸碱吴茱萸次碱薄层色谱高效液相色谱分析
Keywords:
Fufang Weitong JiaonangevodiaminerutaecarpineTLCHPLCanalysis
分类号:
R284.2
DOI:
10.16841/j.issn1003-8450.2017.04.09
摘要:
目的 建立苗药复方胃痛胶囊中吴茱萸的定性与定量分析方法。方法 采用薄层色谱(TLC)法对苗药复方胃痛胶囊中的吴茱萸碱和吴茱萸次碱进行定性分析;采用高效液相色谱(HPLC)法,以Diamonsil C18(4.6 mm×200 mm,5 μm)为色谱柱,乙腈-水-四氢呋喃-冰醋酸(41∶59∶3∶0.1)为流动相,流速1.0 mL/min,检测波长225 nm,柱温30℃,测定苗药复方胃痛胶囊中吴茱萸碱和吴茱萸次碱的含量。结果 所建立的TLC法斑点清晰、分离效果好。含量测定中吴茱萸碱和吴茱萸次碱的回归方程分别为Y=10 211X-6.838(r=0.999 7)和Y=1 931X-8.501(r=0.999 6),说明二者分别在0.098~0.783 mg/mL和0.068~0.544 mg/mL范围内线性关系良好;二者的加样回收率分别为98.98%,97.78%。结论 本法简便可行、重复性好,可用于苗药复方胃痛胶囊中吴茱萸的质量控制。
Abstract:
Objective To establish the qualitative and quantitative analysis methods for testing evodiamine in Fufang Weitong Jiaonang (Miao medicine). Methods Qualitative analysis was made to test evodiamine and rutaecarpine in the capsule by TLC;HPLC was used to determine the contents of evodiamine and rutaecarpine with the column of Diamonsil C18 (4.6 mm×200 mm, 5 μm), moving phase was acetonitrile-waters-tetrahydrofuran-phosphoric acid (41:59:3:0.1), flow rate was 1.0 mL/min, detective wavelength at 225 nm and column temperature at 30℃. Results The spots were clear and separating effect was good. The regression equations of two elements were Y=10 211X-6.838(r=0.999 7)and Y=1 931X-8.501(r=0.999 6), showing a good linearity in the range of 0.098~0.783 mg/mL and 0.068~0.544 mg/mL;the recovery rates of two were 98.98% and 97.78% respectively. Conclusion The method is simple, effective with good repeatability and can be used for quality control of the capsules.

参考文献/References:

[1] 国家食品药品监督管理局.国家中成药标准汇编内科脾胃分册[M].北京:国家食品药品监督管理局,2002:555-556.
[2] 国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:171-172.
[3] 黄志芳,易进海,吴燕,等.吴茱萸药材HPLC含量测定方法的优化[J].中国中药杂志,2011,36(4):478-480.
[4] 张红梅,李国,宋龙,等.贵州和浙江产吴茱萸指纹图谱分析及含量测定[J].中国实验方剂学杂志,2014,20(17):77-81.

备注/Memo

备注/Memo:
收稿日期:2016-09-16。
基金项目:贵州省高层次创新型人才培养项目(遵市科合人才〔2015〕35号);遵义市创新人才团队培养项目(遵市科合〔2016〕7号)。
作者简介:冯华(1977-),男,副主任药师,理学硕士,主要从事药品的检验及新药研究工作。
更新日期/Last Update: 1900-01-01