[1]程芳,靳子明,韩静,等.双波长高效液相色谱法测定羌活感冒颗粒中葛根素和黄芩苷的含量[J].甘肃中医药大学学报,2018,35(03):33-36.[doi:10.16841/j.issn1003-8450.2018.03.09]
 CHENG Fang,JIN Ziming,HAN Jing,et al.Determination of puerarin and baicalin in Qianghuo Ganmao Particle by dual-wavelength HPLC[J].Journal of Gansu University of Chinese Medicine,2018,35(03):33-36.[doi:10.16841/j.issn1003-8450.2018.03.09]
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双波长高效液相色谱法测定羌活感冒颗粒中葛根素和黄芩苷的含量()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
35卷
期数:
2018年03期
页码:
33-36
栏目:
技术与方法
出版日期:
2018-06-25

文章信息/Info

Title:
Determination of puerarin and baicalin in Qianghuo Ganmao Particle by dual-wavelength HPLC
作者:
程芳12 靳子明12 韩静1 贺沙沙1
1. 甘肃中医药大学附属医院药学部, 甘肃 兰州 730020;
2. 甘肃省中药炮制及质控工程技术研究中心, 甘肃 兰州 730020
Author(s):
CHENG Fang12 JIN Ziming12 HAN Jing1 HE Shasha1
1. Pharmacy Department, the Affiliated Hospital of Gansu Uninversity of Chinese Medicine, Lanzhou, Gansu, 730020, China;
2. Research Center of Chinese Herbs Processing & Quality Control Engineering Technology in Gansu Province, Lanzhou, Gansu, 730020, China
关键词:
羌活感冒颗粒葛根素黄芩苷双波长高效液相色谱含量测定
Keywords:
Qianghuo Ganmao Particlepuerarinbaicalindual-wavelengthHPLCcontent determination
分类号:
R284.2
DOI:
10.16841/j.issn1003-8450.2018.03.09
摘要:
目的 建立羌活感冒颗粒中葛根素和黄芩苷的含量测定方法。方法 采用双波长高效液相色谱(HPLC)法测定羌活感冒颗粒中葛根素和黄芩苷的含量,色谱柱为Thermo Hypersil Gold-C18柱(5 μm,4.6 mm×250 mm),流动相为乙腈-0.5%磷酸,梯度洗脱,流速1.0 mL/min,检测波长葛根素为250 nm、黄芩苷为278 nm,柱温30℃。结果 葛根素回归方程为Y=3.9×107X+60 453(r=0.999 6),黄芩苷为Y=3.3×107X+68 288(r=0.999 6),表明葛根素在0.014 88~0.148 80 mg/mL、黄芩苷在0.027 36~0.273 60 mg/mL范围内呈良好的线性关系。葛根素平均回收率为97.95%,黄芩苷为96.33%;葛根素RSD为1.0%,黄芩苷RSD为0.9%。结论 所建立的方法专属性强,重复性好,稳定易行,可用于同时测定羌活感冒颗粒中葛根素和黄芩苷的含量。
Abstract:
Objective To establish content test method for puerarin and baicalin in Qianghuo Ganmao Particle. Methods The content of puerarin and baicalin in Qianghuo Ganmao Particle was determined by dual-wavelength HPLC. The samples were separated on the column of Thermo Hypersil Gold-C18 (5 μm,4.6 mm×250 mm),which moving phase was acetonitrile-phosphoric acid (0.5%). The flow rate was 1.0 mL/min,the detected wavelengths were set at 250 nm and 278 nm respectively and the column temperature was set at 30℃. Results The regression equation of puerarin was Y=3.9×107X+60 453 (r=0.999 6),showing a good linearity in the range of 0.014 88 to 0.148 80 mg/mL;while the regression equation of baicalin was Y=3.3×107X+68 288(r=0.999 6),showing a good linearity in the range of 0.027 36 to 0.273 60 mg/mL. The recovery rates of puerarin and baicalin were 97.95% and 96.33%;while RSD were 1.0% and 0.9% respectively. Conclusion The method is stable and feasible with strong specificity and repeatability,which can be simultaneously used to determine the content of puerarin and baicalin in Qianghuo Ganmao Particle.

参考文献/References:

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[2] 郑修丽,王炯.清热逐风合剂的薄层色谱鉴别[J].甘肃中医,2001,14(6):65.
[3] 夏曦,刘玲.舒筋通络外用颗粒质量标准的研究[J].中国医药导报,2010,7(28):38-41.
[4] 徐晓弘,熊鑫.薄层色谱法鉴别冠脉宁颗粒的研究[J].中国医药导报,2011,8(15):183-184.

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备注/Memo

备注/Memo:
收稿日期:2017-02-27。
作者简介:程芳(1986-),女,中药师,医学硕士,主要从事中药质量标准及中药炮制的研究。
通信作者:靳子明(1966-),男,主任中药师,理学学士,主要从事中药炮制的研究。E-mail:jinziming110@sina.com
更新日期/Last Update: 1900-01-01