[1]李荣科,李岩.岷当归对高脂饲料联合链脲佐菌素诱导的Ⅱ型糖尿病小鼠血清IL-6,IL-22和TNF-α含量的影响[J].甘肃中医药大学学报,2016,33(06):6-9.[doi:10.16841/j.issn1003-8450.2016.06.02]
 LI Rongke,LI Yan.Effect of angelica on the contents of IL-6,IL-22 and TNF-α in serum of mice with type Ⅱ diabetes induced by high-fat diet and streptozotocin[J].Journal of Gansu University of Chinese Medicine,2016,33(06):6-9.[doi:10.16841/j.issn1003-8450.2016.06.02]
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岷当归对高脂饲料联合链脲佐菌素诱导的Ⅱ型糖尿病小鼠血清IL-6,IL-22和TNF-α含量的影响()
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
33卷
期数:
2016年06期
页码:
6-9
栏目:
实验研究
出版日期:
2016-12-25

文章信息/Info

Title:
Effect of angelica on the contents of IL-6,IL-22 and TNF-α in serum of mice with type Ⅱ diabetes induced by high-fat diet and streptozotocin
作者:
李荣科 李岩
甘肃中医药大学基础医学院, 甘肃 兰州 730000
Author(s):
LI Rongke LI Yan
Basic Medicine College, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China
关键词:
岷当归Ⅱ型糖尿病白细胞介素-6白细胞介素-22肿瘤坏死因子-α
Keywords:
angelica in Minxian Countytype ⅡdiabetesIL-6IL-22TNF-α
分类号:
R285.5
DOI:
10.16841/j.issn1003-8450.2016.06.02
摘要:
目的 观察岷当归对高脂饲料(HDF)联合链脲佐菌素(STZ)诱导的Ⅱ型糖尿病小鼠血清白细胞介素-6(IL-6)、白细胞介素-22(IL-22)和肿瘤坏死因子-α(TNF-α)含量的影响。方法 将40只昆明种小鼠随机分为空白组、模型组、防治组和治疗组,每组10只。防治组提前2周灌胃当归水煎液,2周后除空白组外,其他组采用HDF联合STZ腹腔注射造模,造模成功后防治组和治疗组灌胃当归水煎液,其他2组继续灌胃生理盐水,连续灌胃4周。在处死小鼠前1天,各组小鼠进行葡萄糖耐量(OGTT)试验,经药物干预后采用酶联免疫法检测各组小鼠血清IL-6,IL-22和TNF-α的含量。结果 与模型组比较,防治组和治疗组小鼠OGTT值,血清IL-6,IL-22和TNF-α含量均降低,差异均有统计意义(P<0.05);且防治组小鼠OGTT值,血清IL-6,IL-22和TNF-α含量下降更明显,差异均有统计意义(P<0.05)。结论 岷当归可以调节HDF联合STZ诱导的Ⅱ型糖尿病小鼠血清IL-6,IL-22和TNF-α的分泌,缓解Ⅱ型糖尿病小鼠的炎症反应,从而起到调节Ⅱ型糖尿病小鼠免疫平衡的作用。
Abstract:
Objective To observe the effect of angelica in Minxian County on the contents of IL-6,IL-22 and TNF-α in serum of mice with type Ⅱ diabetes induced by high-fat diet and streptozotocin. Methods 40 mice were randomized into blank group,model group,prevention group and treatment group,10 for each. The prevention group was given angelica decoction intragastrically 2 weeks ahead. Except blank control group,other groups were modeled through high-fat diet and streptozotocin. Model mice in prevention group and treatment group were given gastric perfusion of angelica decoction and other two groups were given normal saline,lasting for 4 weeks. OGTT was run in all mice day-ahead before they were killed. The contents of IL-6,IL-22 and TNF-α were detected with ELISA after drug intervention. Results Compared with model group,all contents in prevention group and treatment group declined evidently with statistical significance (P<0.05) and that in prevention group dropped more,there was a difference (P<0.05). Conclusion Angelica in Minxian County can regulate the secretion of IL-6,IL-22 and TNF-α in serum as well as remit the inflammatory reaction. The drug plays a role in adjusting the immune balance in mice with typeⅡdiabetes.

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备注/Memo

备注/Memo:
收稿日期:2015-11-24
作者简介:李荣科(1976-),男,副教授,硕士生导师,医学硕士。研究方向:中医病证结合及动物模型研究。
更新日期/Last Update: 1900-01-01