[1]权春梅.2种方法提取芍花精油成分及其抗氧化活性研究[J].甘肃中医药大学学报,2018,35(06):32-36.[doi:10.16841/j.issn1003-8450.2018.06.08]
 QUAN Chunmei.Research in components and antioxidant activity of essential oil of paeonia by two extraction methods[J].Journal of Gansu University of Chinese Medicine,2018,35(06):32-36.[doi:10.16841/j.issn1003-8450.2018.06.08]
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2种方法提取芍花精油成分及其抗氧化活性研究
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
35卷
期数:
2018年06期
页码:
32-36
栏目:
技术与方法
出版日期:
2018-12-25

文章信息/Info

Title:
Research in components and antioxidant activity of essential oil of paeonia by two extraction methods
作者:
权春梅12
1. 亳州职业技术学院药学院, 安徽 亳州 236800;
2. 安徽中医药科学院亳州中医药研究所, 安徽 亳州 236800
Author(s):
QUAN Chunmei12
1. School of Pharmacy, Bozhou Vocational and Technical College, Bozhou, Anhui, 236800, China;
2. Bozhou Institute of TCM, Anhui Academy of TCM, Bozhou, Anhui, 236800, China
关键词:
芍花精油超临界CO2流体萃取法水蒸气蒸馏法抗氧化活性
Keywords:
essential oil of paeoniasupercritical CO2 fluid extractionsteam distillationantioxidant activity
分类号:
R284.2
DOI:
10.16841/j.issn1003-8450.2018.06.08
摘要:
目的 考察超临界CO2流体萃取法和水蒸气蒸馏法提取的芍花精油成分及其抗氧化活性。方法 采用超临界CO2流体萃取法和水蒸气蒸馏法提取芍花中的精油,通过气相色谱-质谱联用技术(GC-MS)对所提芍花精油进行成分分析,并采用1,1-二苯基-2-三硝基苯肼(DPPH)法和2,2’-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)法考察2种方法提取的芍花精油的抗氧化活性强弱。结果 超临界CO2流体萃取法提取的芍花精油分离鉴定出了10种主要化学成分,主要成分是烯醇,亦含有少量的醇、苯甲酸和酮等成分;而水蒸气蒸馏法提取的芍花精油分离鉴定出了8种主要化学成分,主要成分是烷烃,含有少量的醇和酮等成分,其中鉴定出了常用的、抗氧化能力较强的2,6-二叔丁基-4-甲基苯酚(BHT)。2种方法提取的芍花精油对DPPH自由基和ABTS自由基均有一定的清除作用。2种方法提取的芍花精油对DPPH自由基的清除率比较差异无统计学意义(P>0.05);水蒸气蒸馏法提取的芍花精油对ABTS自由基的清除率大于超临界CO2流体萃取法,差异有统计学意义(P<0.05)。结论 超临界CO2流体萃取法和水蒸气蒸馏法提取的芍花精油成分及抗氧化活性均不同,为芍花精油的成分及其抗氧化活性研究提供了理论基础,同时为优选芍花精油的提取方法提供了试验依据。
Abstract:
Objective To investigate the components and antioxidant activity of the essential oil of paeonia by supercritical CO2 fluid extraction and steam distillation. Methods We used supercritical CO2 fluid extraction and steam distillation to extract the essential oil of paeonia, and used gas chromatography-mass spectrography (GC-MS) to analyse the components of it, and also used DPPH method and ABTS methods to investigate the antioxidant activity of it. Results Totally ten components were identified from it by the supercritical CO2 fluid extraction, with the main component enol and a small amount of the components alcohol, benzoic acid and ketone etc. Totally eight components were identified from it by the steam distillation, with the main component alkane and a small amount of alcohol and ketone etc. Particularly, BHT which is a common and strong antioxidant was identified. The essential oil of paeonia extracted by the two methods had a scavenging effect on DPPH free radical and ABTS free radical.There was no statistically significant differences between the scavenging rates of DPPH free radical in essential oil of paeonia extracted by the two methods (P>0.05);the scavenging rate of ABTS free radical of essential oil extracted by steam distillation was higher than that by supercritical CO2 fluid extraction, there was statistically significant diffe-rence (P<0.05). Conclusion The components and antioxidant activity of essential oil of paeonia extracted by supercritical CO2 fluid extraction and steam distillation vary from each other, which provides a theoretical basis for the components and antioxidant research as well as the optimal extraction of essential oil of paeonia.

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备注/Memo

备注/Memo:
收稿日期:2018-01-10。
基金项目:安徽省高等学校自然科学研究项目(KJ2016A496)。
作者简介:权春梅(1984-),女,实验师,工程硕士,主要从事中药有效成分提取及中药质量控制。
更新日期/Last Update: 1900-01-01