[1]张薇薇,杨雅丽,樊景春,等.EDC/NHS交联剂在新型生物材料磁小体修饰中的应用[J].甘肃中医药大学学报,2019,36(01):1-5.[doi:10.16841/j.issn1003-8450.2019.01.01]
 ZHANG Weiwei,YANG Yali,FAN Jingchun,et al.Application of EDC/NHS cross-linking agent in neotype biomaterial magnetosome modification[J].Journal of Gansu University of Chinese Medicine,2019,36(01):1-5.[doi:10.16841/j.issn1003-8450.2019.01.01]
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EDC/NHS交联剂在新型生物材料磁小体修饰中的应用
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
36卷
期数:
2019年01期
页码:
1-5
栏目:
论著
出版日期:
2019-02-25

文章信息/Info

Title:
Application of EDC/NHS cross-linking agent in neotype biomaterial magnetosome modification
作者:
张薇薇1 杨雅丽1 樊景春2 吴建军2 殷晓春2 李海龙1 舍雅莉3 王琳4
1. 甘肃中医药大学临床医学院, 甘肃 兰州 730000;
2. 甘肃中医药大学公共卫生学院, 甘肃 兰州 730000;
3. 甘肃中医药大学基础医学院, 甘肃 兰州 730000;
4. 甘肃中医药大学附属医院放射科, 甘肃 兰州 730020
Author(s):
ZHANG Weiwei1 YANG Yali1 FAN Jingchun2 WU Jianjun2 YIN Xiaochun2 LI Hailong1 SHE Yali3 WANG Lin4
1. Clinical Medical College, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China;
2. School of Public Health, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China;
3. Basic Medicine College, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China;
4. Department of Radiology, Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou, Gansu, 730020, China
关键词:
1-乙基-3-(3-二甲基氨丙基)-碳化二亚胺(EDC)N-羟基琥珀酰亚胺(NHS)交联剂磁小体纳米技术
Keywords:
1-ethyl-3-(3-dimethylaminopropyl)-carbonized diimine(EDC)N-hydroxysuccinimide(NHS)cross-linking agentmagnetosomenanotechnology
分类号:
R318.08
DOI:
10.16841/j.issn1003-8450.2019.01.01
摘要:
目的 使用1-乙基-3-(3-二甲基氨丙基)-碳化二亚胺(EDC)/N-羟基琥珀酰亚胺(NHS)交联剂制备基于磁小体的主动靶向纳米颗粒。方法 使用EDC/NHS作为交联剂,在超声振荡孵育下交联细胞色素c适体修饰的磁小体及甲氧基-聚乙二醇-羧基(methoxy-PEG-carboxymethyl,分子量2 000 Da,mPEG-COOH2000),得到终产物聚乙二醇化的靶向细胞色素c适体的磁小体纳米颗粒PEG-cBMP。结果 透射电镜表征结果显示终产物PEG-cBMP的包膜较纯化磁小体的包膜明显增厚,约10 nm,说明在交联剂的作用下修饰磁小体成功。结论 采用EDC/NHS交联剂修饰磁小体这种生物源性纳米材料是可行的。
Abstract:
Objective To prepare active targeted nanoparticles based on magnetosomes using 1-ethyl-3-(3-dimethylaminopropyl)-carbonized diimine(EDC)/N-hydroxysuccinimide(NHS) cross-linking agents.Methods Using EDC/NHS as crosslinking agent,PEG-cBMP was prepared by crosslinking cytochrome c-aptamer modified magnetosomes and methoxy-PEG-carboxymethyl(molecular weight 2 000 Da,mPEG-COOH2000) under ultrasonic oscillation incubation.Results The results of transmission electron microscopy characterization showed that the capsule of the end product PEG-cBMP was about 10 nm,significantly thicker than that of purified magnetosomes,indicating that the modification of magnetosomes under the action of cross-linking agent was successful.Conclusion It is feasible to modify magnetosomes with EDC/NHS cross-linking agent as biogenic nanomaterials.

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备注/Memo

备注/Memo:
收稿日期:2018-10-17。
基金项目:2017年国家留学基金委西部地区人才培养特别项目(201708625044);2016年甘肃中医药大学引进人才科研启动基金项目(2016YJRC-02);2016年甘肃省高等学校科研项目(2016B-059);2016年甘肃省卫生行业科研计划项目(GSWSKY2016-25)。
作者简介:张薇薇(1980-),女,副教授,医学博士,主要从事医学影像诊断教学及分子影像学研究。
更新日期/Last Update: 1900-01-01