[1]刘雪枫,梁希晨,岳栋芳,等.高效液相色谱法测定当归芍药散不同极性部位中阿魏酸和芍药苷的含量[J].甘肃中医药大学学报,2019,36(01):33-38.[doi:10.16841/j.issn1003-8450.2019.01.07]
 LIU Xuefeng,LIANG Xichen,YUE Dongfang,et al.Content determination of ferulic acid and paeoniflorin of different polar parts in Danggui Shaoyao San(当归芍药散) by HPLC[J].Journal of Gansu University of Chinese Medicine,2019,36(01):33-38.[doi:10.16841/j.issn1003-8450.2019.01.07]
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高效液相色谱法测定当归芍药散不同极性部位中阿魏酸和芍药苷的含量
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
36卷
期数:
2019年01期
页码:
33-38
栏目:
技术与方法
出版日期:
2019-02-25

文章信息/Info

Title:
Content determination of ferulic acid and paeoniflorin of different polar parts in Danggui Shaoyao San(当归芍药散) by HPLC
作者:
刘雪枫12 梁希晨2 岳栋芳2 钱倩13 刘东玲1
1. 甘肃省中药药理与毒理重点实验室, 甘肃 兰州 730000;
2. 甘肃中医药大学药学院, 甘肃 兰州 730000;
3. 甘肃省人民医院药剂科, 甘肃 兰州 730000
Author(s):
LIU Xuefeng12 LIANG Xichen2 YUE Dongfang2 QIAN Qian13 LIU Dongling1
1. The Key Laboratory for TCM Pharmacology and Toxicology in Gansu Province, Lanzhou, Gansu, 730000, China;
2. School of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, China;
3. Pharmaceutical Preparation Section, Gansu Provincial the People's Hospital, Lanzhou, Gansu, 730000, China
关键词:
当归芍药散阿魏酸芍药苷高效液相色谱法含量测定
Keywords:
Danggui Shaoyao San(当归芍药散)ferulic acidpaeoniflorinHPLCcontent determination
分类号:
R284.2
DOI:
10.16841/j.issn1003-8450.2019.01.07
摘要:
目的 建立当归芍药散不同极性部位中阿魏酸和芍药苷的含量测定方法。方法 采用高效液相色谱(HPLC)法测定当归芍药散不同极性部位中阿魏酸和芍药苷的含量,色谱柱为Kromasil 100-5-C18柱(4.6 mm×250.0 mm),流动相为甲醇:0.2%磷酸溶液,梯度洗脱,流速1 mL/min,阿魏酸、芍药苷的检测波长分别为320,230 nm,进样量10 μL,柱温30℃。结果 阿魏酸和芍药苷的回归方程分别为Y=3.53×104X-4.83×102r=0.999 0),Y=1.50×104X-2.51×104r=0.999 9),线性范围分别为6.076~303.800 μg/mL和7.448~372.400 μg/mL;方法学考察中精密度、稳定性和重复性均符合要求,加样回收率的RSD分别为1.2%和1.1%。含量测定结果显示,除醇提物的水相部分未检出阿魏酸外,其他部分中均存在阿魏酸和芍药苷,且水煎液中阿魏酸、芍药苷含量均最高。结论 该法简便、准确、分离效果好,可用于当归芍药散不同极性部位中阿魏酸、芍药苷的含量测定。
Abstract:
Objective To establish a content determination method for ferulic acid and paeoniflorin of different polar parts in Danggui Shaoyao San(当归芍药散). Methods HPLC was used with Kromasil 100-5-C18 column (4.6 mm×250.0 mm). The mobile phase was methanol:0.2% phosphoric acid solution with gradient elution. The flow rate was 1.0 mL/min,the detection wavelength of ferulic acid and paeoniflorin was 320 nm and 230 nm,respectively. The column temperature was 30℃,and sample size was 10 μL. Results The regression equations of ferulic acid and paeoniflorin were Y=3.53×104X-4.83×102(r=0.999 0) and Y=1.50×104X-2.51×104(r=0.999 9),respectively. The linearity ranged from 6.076 to 303.800 μg/mL for ferulic acid,and from 7.448 to 372.400 μg/mL for paeoniflorin. The precision,stability and repeatability of methodological investigation meet the requirements. The RSD of sample recovery rate was 1.2% and 1.1%,respectively. The results of content determination showed that ferulic acid and paeoniflorin existed in other parts except in the aqueous phase of methanol extract,and the content of ferulic acid and paeoniflorin in water decoction was the highest.Conclusion The method is simple and accurate,with good separating effect. It can be used for content determination of ferulic acid and paeoniflorin of different polar parts in Danggui Shaoyao San.

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备注/Memo

备注/Memo:
收稿日期:2018-07-09。
基金项目:甘肃省中药药理与毒理重点实验室开放基金项目(ZDSYS-KJ-2015-004);甘肃省中药药理与毒理重点实验室自主基金项目(ZDSYS-ZZ-2015-003);甘肃中医药大学中青年基金项目(2305016601)。
作者简介:刘雪枫(1987-),女,讲师,医学硕士,主要从事中药药理学的教学与实验研究。
更新日期/Last Update: 1900-01-01