[1]王惠珍,陆国弟,胡茹英,等.聚乙二醇-6000模拟干旱胁迫下硅对蒙古黄芪种子萌发特性和幼苗质量的影响[J].甘肃中医药大学学报,2020,37(01):31-36.[doi:10.16446/j.cnki.1001-1994.2020.01.007]
 WANG Huizhen,LU Guodi,HU Ruying,et al.Effects of silicon on seed germination characteristics and seedling quality of Astragalus membranaceus (Fish) Bge.var.mongholicus(Bunge.) Hsiao under PEG-6000 simulated drought stress[J].Journal of Gansu University of Chinese Medicine,2020,37(01):31-36.[doi:10.16446/j.cnki.1001-1994.2020.01.007]
点击复制

聚乙二醇-6000模拟干旱胁迫下硅对蒙古黄芪种子萌发特性和幼苗质量的影响
分享到:

《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
37卷
期数:
2020年01期
页码:
31-36
栏目:
论著
出版日期:
2020-02-25

文章信息/Info

Title:
Effects of silicon on seed germination characteristics and seedling quality of Astragalus membranaceus (Fish) Bge.var.mongholicus(Bunge.) Hsiao under PEG-6000 simulated drought stress
作者:
王惠珍 陆国弟 胡茹英 陈红刚 王引权
甘肃中医药大学药学院, 甘肃 兰州 730101
Author(s):
WANG Huizhen LU Guodi HU Ruying CHEN Honggang WANG Yinquan
School of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730101, China
关键词:
蒙古黄芪聚乙二醇-6000干旱胁迫萌发特性幼苗质量
Keywords:
Astragalus membranaceus(Fish) Bge.var.mongholicus(Bunge.) HsiaoPEG-6000drought stresssilicongermination characteristicsseedling quality
分类号:
S567.239
DOI:
10.16446/j.cnki.1001-1994.2020.01.007
摘要:
目的 研究干旱胁迫下硅对蒙古黄芪种子萌发特性和幼苗质量的影响,探索硅在提高蒙古黄芪抗旱性方面的作用。方法 采用二因素完全随机设计及培养皿沙培试验,以聚乙二醇(PEG)-6000模拟不同程度的干旱胁迫,并加入硅(浓度分别为0,3,6 mmoL/L),测定各处理下种子萌发特性和幼苗质量。结果 随PEG-6000模拟浓度的增加,各测定指标均呈不同程度的降低。1)重度胁迫下发芽率、发芽势和发芽指数较正常供水分别降低48.7%,76.1%,75.1%,硅可明显缓解以上指标的下降趋势;2)重度胁迫下全株长、子叶长、子叶宽、鲜重较正常供水和中度胁迫明显降低(P<0.05),中度胁迫与正常供水比较差异无统计学意义(P>0.05),而硅的加入除全株长外,对其他指标均无明显影响;3)中度胁迫下叶片相对含水量较正常供水降低3.5%,重度胁迫较中度胁迫和正常供水分别降低7.6%,10.8%,6 mmoL/L的硅可使重度胁迫下的叶片相对含水量明显升高(P<0.05);4)在重度胁迫下,叶绿素a含量和叶绿素a/b值较正常供水分别降低13.6%,28.0%,叶绿素b变化不明显,硅可使叶绿素含量呈不同程度的增加。结论 适当干旱胁迫对蒙古黄芪种子萌发和幼苗生长无显著影响,重度胁迫可显著抑制种子萌发、幼苗生长和叶绿素的合成;硅能干预重度干旱胁迫对黄芪种子萌发和幼苗生长的抑制作用,为蒙古黄芪的规范化生产和硅肥的合理使用提供了试验依据。
Abstract:
Objective To study the effect of silicon on seed germination characteristics and seedling quality of Astragalus membranaceus(Fish) Bge.var.mongholicus (Bunge.) Hsiao under drought stress,and to explore the role of silicon in improving drought resistance of Astragalus membranaceus(Fish) Bge.var.mongholicus(Bunge.) Hsiao.Methods Two-factor random design and Petri dish sand culture experiment were used to simulate drought stress in different degrees with PEG-6000,and silicon (the concentration was 0,3 and 6 mmol/L,respectively.) was added to determine seed germination characteristics and seedling quality under different treatments. Results With the increase of simulated concentration of PEG-6000,the determinative indexes showed a decreasing trend in varying degrees.1)The germination rate,germination potential and germination index under severe stress were 48.7%,76.1% and 75.1% lower than CK,respectively. Silicon significantly alleviated the downward trend.2)The whole plant length,cotyledon length,cotyledon width and fresh weight of plant under severe stress were significantly lower than those under CK and moderate stress(P<0.05),but there was no significant difference between moderate stress and CK(P>0.05),while the addition of silicon had no significant effects on other indexes except the whole plant length. 3)Relative water content of leaves under moderate stress was 3.5% lower than CK,while that under severe stress was 7.6% and 10.8% lower than that under moderate stress and CK,respectively. And 6 mmoL/L of silicon made the relative water content of leaves under severe stress increase significantly(P<0.05).4)Under severe stress,content of chlorophyll a and chlorophyll a/b decreased by 13.6% and 28.0% respectively compared with CK,but chlorophyll b did not change significantly. Silicon increased chlorophyll content in different degrees. Conclusion Appropriate drought stress has no significant effect on seed germination and seedling growth of Astragalus membranaceus(Fish) Bge.var.mongholicus(Bunge.) Hsiao. Severe stress significantly inhibits seed germination,seedling growth and chlorophyll synthesis. Silicon can improve the inhibition of severe drought stress on seed germination and seedling growth of Astragalus membranaceus(Fish) Bge.var.mongholicus(Bunge.) Hsiao,which provides experimental basis for standardized production of Astragalus membranaceus(Fish) Bge.var.mongholicus(Bunge.) Hsiao and rational use of silicon fertilizer.

参考文献/References:

[1] 国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:283.
[2] 石玉.外源硅对番茄幼苗水分胁迫伤害的缓解效应及机理研究[D].杨凌:西北农林科技大学,2014.
[3] 张文晋,解植彩,张新慧,等.硅对不同程度干旱胁迫下甘草种子萌发和幼苗生长的影响[J].世界科学技术-中医药现代化,2016,18(12):2125-2131.
[4] 曹逼力,徐坤,石健,等.硅对番茄生长及光合作用与蒸腾作用的影响[J].植物营养与肥料学报,2013,19(2):354-360.
[5] 明东风.硅对水分胁迫下水稻生理生化特性、亚显微结构及相关基因表达的调控机制研究[D].杭州:浙江大学,2012.
[6] 余群.干旱胁迫下硅肥对草地早熟禾苗期生长发育的影响[D].兰州:兰州大学,2014.
[7] 吴淼,刘信宝,丁立人,等.PEG模拟干旱胁迫下硅对紫花苜蓿萌发及生理特性的影响[J].草地学报,2017,25(6):1258-1264.
[8] 王耀晶,马聪,张薇,等.干旱胁迫下硅对草莓生长及生理特性的影响[J].核农学报,2013,27(5):703-707.
[9] 郑世英,郑晓彤,耿建芬,等.硅对干旱胁迫下野生大豆幼苗生长和生理特性的影响[J].大豆科学,2018,37(2):263-267.
[10] 李季平,古红梅,吴诗光,等.聚乙二醇(PEG)处理对小麦萌发种子生理生化特性的影响[J].河南农业科学,2002(6):4-6.
[11] 周虹,范巧佳,郑顺林,等.春季水分胁迫对川芎叶片相对含水量及保护酶活性的影响[J].中国中药杂志,2009,34(2):132-137.

备注/Memo

备注/Memo:
收稿日期:2019-03-27。
基金项目:现代农业产业技术体系建设专项资金资助项目(CARS-21);西北中藏药协同创新中心2015年度重点项目(XBXT2015-4);甘肃省自然科学基金项目(177R5RA166)。
作者简介:王惠珍(1974-),女,副教授,农学博士,主要从事药用植物生理生态研究。
更新日期/Last Update: 1900-01-01