[1]赵文秀,马骏,王志晓,等.铭悦茜白片对小鼠出血、凝血、血浆复钙时间及血小板计数的影响[J].甘肃中医药大学学报,2020,37(01):37-40.[doi:10.16446/j.cnki.1001-1994.2020.01.008]
 ZHAO Wenxiu,MA Jun,WANG Zhixiao,et al.Effects of Mingyue Qianbai Pian(铭悦茜白片) on bleeding,clotting,plasma recalcification time and platelet count in mice[J].Journal of Gansu University of Chinese Medicine,2020,37(01):37-40.[doi:10.16446/j.cnki.1001-1994.2020.01.008]
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铭悦茜白片对小鼠出血、凝血、血浆复钙时间及血小板计数的影响
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《甘肃中医药大学学报》[ISSN:1003-8450/CN:62-1062/R]

卷:
37卷
期数:
2020年01期
页码:
37-40
栏目:
实验研究
出版日期:
2020-02-25

文章信息/Info

Title:
Effects of Mingyue Qianbai Pian(铭悦茜白片) on bleeding,clotting,plasma recalcification time and platelet count in mice
作者:
赵文秀12 马骏12 王志晓12 李文基12 刘兴国3
1. 甘肃中医药大学药学院, 甘肃 兰州 730101;
2. 国家中医药管理局中医药科研三级实验室中药药理实验室, 甘肃 兰州 730000;
3. 兰州市食品药品检验所, 甘肃 兰州 730050
Author(s):
ZHAO Wenxiu12 MA Jun12 WANG Zhixiao12 LI Wenji12 LIU Xingguo3
1. School of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730101, China;
2. Pharmacology Laboratory of Chinese Materia Medica, Tertiary-level Laboratory for Traditional Chinese Medicine Scientific Research of National Administration of Traditional Chinese Medicine, Lanzhou, Gansu, 730000, China;
3. Lanzhou Institute for Food and Drug Control, Lanzhou, Gansu, 730050, China
关键词:
铭悦茜白片出血时间凝血时间血浆复钙时间血小板计数小鼠实验研究
Keywords:
Mingyue Qianbai Pian(铭悦茜白片MQP)bleeding time(BT)clotting time(CT)plasma recalcification time(PRT)platelet countmiceexperimental study
分类号:
R285.5
DOI:
10.16446/j.cnki.1001-1994.2020.01.008
摘要:
目的 观察铭悦茜白片对小鼠出血时间(BT)、凝血时间(CT)、血浆复钙时间(PRT)及血小板计数的影响,探讨其止血作用及作用机制。方法 采用随机数字表法将200只BALB/c小鼠分为4大组,每组50只,再将每大组随机分为空白组,阳性对照组,铭悦茜白片高、中、低剂量组5组,每组10只,各组分别予0.5%羧甲基纤维素钠(CMC-Na)及云南白药(0.667 g/kg)、铭悦茜白片(1.80,0.90,0.45 g/kg)混悬液0.02 mL/g灌胃,每日1次,连续灌胃7 d。末次给药后分别采用断尾法、毛细玻管法、试管法测定各组小鼠BT、CT及PRT,采用高倍显微镜观察各组小鼠血小板计数。结果 与空白组比较,阳性对照组,铭悦茜白片高、中剂量组BT、CT及PRT均明显缩短,铭悦茜白片低剂量组CT明显缩短,差异有统计学意义(P<0.05或P<0.01);与铭悦茜白片高剂量组比较,铭悦茜白片低剂量组BT及PRT均明显延长,铭悦茜白片中、低剂量组CT均明显延长,差异有统计学意义(P<0.05)。与空白组比较,阳性对照组,铭悦茜白片高、中、低剂量组血小板计数均明显增多,差异有统计学意义(P<0.05或P<0.01);与铭悦茜白片高剂量组比较,铭悦茜白片低剂量组血小板计数明显减少,差异有统计学意义(P<0.05)。结论 铭悦茜白片可明显缩短小鼠BT、CT及PRT,升高血小板计数,具有明显的止血作用,其止血作用机制可能与增加血小板数量有关。
Abstract:
Objective To observe the effects of Mingyue Qianbai Pian(铭悦茜白片,MQP) on bleeding time(BT),clotting time(CT),plasma recalcification time(PRT) and platelet count in mice,and discuss its hemostatic effect and mechanism.Methods Totally 200 BALB/c mice were divided into 4 big groups,with 50 in each. Each big group was randomly divided into 5 subgroups,including blank group,positive control group,and MQP high,medium and low dose group,with 10 in each. Each subgroup was given 0.5% sodium carboxymethylcellulose (CMC-Na),Yunnan Baiyao(0.667 g/kg) and MQP(1.80,0.90,0.45 g/kg) 0.02 mL/g suspension through administration by gavage,once per day,administration by gavage for 7 consecutive days. After the last administration,BT,CT and PRT in mice of each subgroup were determined by tail-cutting method,glass capillary method and test tube method,respectively. Platelet counts of mice in each group was observed by high power microscope. Results Compared with the blank group,BT,CT and PRT of the positive control group and MQP high and medium dose group significantly shortened(P<0.05),CT of MQP low dose group significantly shortened,and the difference was statistically significant(P<0.05 or P<0.01). Compared with MQP high dose group,BT and PRT of MQP low dose group significantly prolonged, CT of MQP medium and low dose group significantly prolonged,and the difference was statistically significant(P<0.05). Compared with the blank group,platelet count in the positive control group,MQP high,medium and low dose group increased significantly,and the difference was statistically significant(P<0.05 or P<0.01). Compared with MQP high dose group,platelet count in MQP low dose group significantly decreased,and the difference was statistically significant(P<0.05).Conclusion MPQ can significantly shorten BT,CT and PRT in mice,and increase platelet count,indicating that MQP has marked hemostatic effect and its hemostatic mechanism may be related to increasing platelet count.

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备注/Memo

备注/Memo:
收稿日期:2019-03-07。
作者简介:赵文秀(1993-),女,在读硕士研究生。研究方向:中药药理与毒理。
通信作者:马骏(1966-),男,回族,教授,硕士生导师,理学硕士。研究方向:中药药理与毒理。E-mail:850304549@qq.com
更新日期/Last Update: 1900-01-01